African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12481

Full Length Research Paper

Thermostable α-amylase production by Bacillus firmus CAS 7 using potato peel as a substrate

S. Elayaraja*, T. Velvizhi, V. Maharani, P. Mayavu, S. Vijayalakshmi and T. Balasubramanian
Faculty of Marine Sciences, CAS in Marine Biology, Annamalai University, Parangipettai 608502, India.
Email: [email protected]

  •  Accepted: 18 August 2011
  •  Published: 19 September 2011

Abstract

 

Thermostable alkaline α-amylase producing bacterium Bacillus firmus CAS 7 strain isolated from marine sediment of Parangipettai coast grew maximally in both shake flasks. Potato peel was found to be a superior substrate for the production of α-amylase at 35°C, pH 7.5 and 1.0% of substrate concentrations. Under optimal conditions, B. firmus produced 676 U/ml of α-amylase which was optimally active at 50°C and pH 9.0.

 

Key words: α-Amylase, potato peel substrate, Bacillus firmus CAS 7, thermostable, marine.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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