African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

An efficient hairy root culture system for Withania somnifera (L.) Dunal

Ganeshan Sivanandhan
  • Ganeshan Sivanandhan
  • Department of Biotechnology and Genetic Engineering, School of Biotechnology, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India.
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Natesan Selvaraj
  • Natesan Selvaraj
  • Periyar E. V. R College (Autonomous), Tiruchirappalli-620 023, Tamil Nadu, India.
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Andy Ganapathi
  • Andy Ganapathi
  • Department of Biotechnology and Genetic Engineering, School of Biotechnology, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India.
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Markandan Manickavasagam*
  • Markandan Manickavasagam*
  • Department of Biotechnology and Genetic Engineering, School of Biotechnology, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India.
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  •  Received: 26 August 2014
  •  Accepted: 22 September 2014
  •  Published: 22 October 2014

Abstract

Withania somnifera is an important aromatic medicinal plant and possesses wide array of pharmacological properties. In the present investigation, an improved version of hairy root culture system was developed by optimizing various transformation parameters such as type of explant, concentration of acetosyringone, Agrobacterium types and co-cultivation period. Between the leaf and cotyledon explants and two Agrobacterium rhizgenes strains (R1000 and A4) tested, leaf explants infected with R1000 and cocultured for five days on MS basal half strength medium in the presence of acetosyringone (100 µM) attained a higher frequency (88%) of hairy root induction. By adopting this protocol, we could utilize the hairy root culture for industrial scale production of withanolides.

 

Key words: Leaf explant, Agrobacterium rhizogenes, Withania somnifera, co-cultivation period, acetosyringone.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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