Abstract

Microbial conversion of corn stover by white rot fungi has the potential to increase its ligninolysis and nutritional value, thereby transforming it into protein-enriched animal feed. Response surface methodology was applied to optimize conditions for the production of lignocellulolytic enzymes by Trametes versicolor during solid-state fermentation of corn stover, as well as enhance ligninolysis and increase the crude protein content. The effects of an additional carbon source (glucose), copper sulfate (CuSO₄) and initial moisture content on lignocellulolytic enzymes, changes in chemical constituents and the crude protein content of corn stover were investigated. T. versicolor produced high laccase, moderate xylanase, and low CMCase activity, whereas neither LiP nor MnP activity was detected. An overall 20-fold increase in laccase activity (45.1 U/g corn stover) was achieved under the optimized conditions. The maximum degradation of lignin and hemicellulose was up to 34.8 and 21.9%, respectively. However, the maximum cellulose loss was less than 10.5%. The crude protein content of the fermented corn stover was doubled under the optimized conditions. Therefore, T. versicolor is a potential organism for laccase production using solid-state fermentation, as well as the simultaneous enhancement of delignification and improvement of the crude protein content in corn stover.

Key words: Corn stover, central composite design, laccase, ligninolysis, Trametes versicolor, crude protein.

Abbreviation

SSF, Solid-state fermentation.