August 2011
Thidiazuron: A multi-dimensional plant growth regulator
Thidiazuron (TDZ) has gained a considerable attention during past decades due to its efficient role in plant cell and tissue culture. Wide array of physiological responses were observed in response to TDZ-application in different plant species. TDZ has shown both auxin and cytokinin like effects, although, chemically, it is totally different from commonly used auxins and cytokinins. A number of biological...
August 2011
Expression of recombinant Streptokinase from local Egyptian Streptococcus sp. SalMarEg
Streptokinase (SK) is a therapeutically important thrombolytic agent. Cardiovascular disease is the first cause of adult death worldwide. In Egypt about 13% of the population die every year due to ischemic heart disease. In spite of this fact, there is no local production of cardiovascular therapeutics. We reported for the first time the expression of a recombinant SK from a local Streptococcus strain. When...
August 2011
CatSper ion channels: Bioinformatics analysis in Homo sapiens
Due to the availability of huge amount of molecular biology data, our main focuswas to determine the protein structures, functions and their role in different molecular pathways. The 3-D structure prediction of protein is important in medicine and biotechnology. Molecular docking not only finds the interaction between proteins but also the accurate models of energy of these interacting proteins and...
August 2011
Characterization of novel developed expressed sequence tag (EST)-derived simple sequence repeat (SSR) markers and their application in diversity analysis of eggplant
A total of 101,270 eggplant expressed sequence tag (EST) sequences at public databases were used to search for simple sequence repeats (SSRs) and 405 potential SSR loci were identified from 388 sequences. The highest proportion (34.07%, 138) was represented by trinucleotide, followed by dinucleotide (19.51%, 79) and hexanucleotide (15.8%, 64). Among the dinucleotide repeats, AG/CT was the most...
August 2011
An effective method for RNA extraction from grapevine berry skins
RNA extraction is the foundation of modern molecular biology. It is difficult to extract high quality RNA from grapevine tissues, especially in cases where polysaccharides and polyphenols are rich. In this paper, the modified cetyltrie thylammnonium bromide (CTAB), sodium dodecyl sulphate (SDS) and guanidine isothiocyanate methods were applied to extract high purity and integrity RNA from grapevine green and mature...
August 2011
Effects of different mowing dates of plant top on tuber yield of Jerusalem artichoke (Helianthus tuberosus L.)
This study was conducted to determine the effects of different mowing dates of plant top on tuber yield of Jerusalem artichoke (Helianthus tuberosus L) during 2005 and 2006 growing seasons. The experiment was designed according to the “split plots on randomized complete block” experimental design with three replications. Three different mowing dates [a: August 18 (before flowering), b: September 21...
August 2011
Interactions of a hairy vetch-corn rotation and P fertilizer on the NPK balance in an upland red soil of the Yunnan plateau
A field experiment was conducted on an infertile red soil under a hairy vetch (Vicia villosa Roth) and corn (Zea mays L.) rotation system in a highland area of Yunnan Province, China. Effects of phosphate (P) fertilization, combined with hairy vetch returned to the soil, on crop yield and soil fertility were studied, and the balances of nitrogen (N), phosphorus (P) and potassium (K) in the rotation system were...
August 2011
Emergence and seedling growth of five forage legume species at various burial depth and two light levels
A field study compared the seedling emergence and structure of five forage legumes (Trifolium repens L., Medicago falcata L., Melilotus suaveolens Ledeb,Medicago sativa L. and Lespedeza davurica Schindler) at five planting depths (1, 2, 4, 6 and 8 cm) and two light levels (full light and shade) on the 21st day after planting. As expected, increaseing depth lowered and slowed...
August 2011
Molecular characterization of two isolates of sweet potato leaf curl virus infecting Ipomoea indica in China
Morning glory (Ipomoea indica) plants showing yellow mosaic and vein yellowing symptoms were collected from Jiangsu and Yunnan province of China. The amplified DNA-A components of two isolates named JS1 and Y338 were comprised of 2827 and 2801 nucleotides, respectively, in length with genome organization similar to that of begomoviruses. Complete nucleotide sequence revealed the presence of two ORFs (AV1 and AV2)...
August 2011
The effect of drought stress and exogenous abscisic acid on growth, protein content and antioxidative enzyme activity in saffron (Crocus sativus L.)
This study was carried out to investigate the effect of drought and abscisic acid (ABA) on growth, protein content and antioxidative enzyme activity in leaves, roots and corms of Crocus sativus L. A comparative study of sample and control showed that leaf relative water content (RWC), length and number of leaves decreased due to drought and ABA when compared to control. Drought and ABA were effective in...
August 2011
The effects of prolonged vegetative reproduction of the two Iranian olive cv. tree cultivars (Dezful Baghmalek and Dezful Safiabad) on morphological traits
Somaclonal variation of the two Iranian olive cultivars named Dezful Baghmalek (DB) and Dezful Safiabad (DS) during the long-term propagation among 7 subcultures were evaluated. Morphological traits such as leaf length, leaf width, number of leaves on shoot, the length of shoots, internode distance and rooting percentage were measured. The study results showed that DB affected somaclonal variations more than the DS...
August 2011
Identification of appropriate tools of information and communication technologies (ICT) in the improvement of food security of Iran's rural households
Access to sufficient and desirable food is one of the principles of any developing and healthy society. One of the important means for attainment of food security is information and communication technologies (ICT). The purpose of the research was to identify appropriate tools of ICT in improving food security of Iran's rural households. The population for this research was 253 extension experts from eight...
August 2011
Effect of salinity and inoculation with Azosprillium on carbohydrate production, nitrogen status and yield of barley
In order to evaluate the effect of Azosprillium brasilense inoculation on common barley grown in saline condition, a greenhouse experiment was conducted in Azad University, branch of Eghlid, Iran. The experimental design was a factorial based on complete randomized design with four replications. The first factor comprised of four saline water treatments (1 as control, 5, 10 and 15 ds mˉ¹) and...
August 2011
Antiviral activity of Conyza canadensis (L.) Cronquist extracts grown in Tunisia
Ethyl acetate, chloroform, butanol and methanol extracts of the aerial parts ofConyza Canadensis L. Cronquist were investigated for their antiviral activity against human cytomegalovirus (HCMV) AD-169 and Cox-B3 viruses by modification of the widely used shell-vial assay. The results showed that butanol and methanol extracts had the most potent antiviral activity against HCMV and Cox-B3...
August 2011
Characterization of copper resistant ciliates: Potential candidates for consortia of organisms used in bioremediation of wastewater
Metals are environmental pollutants of major concern due to their ecological, sanitary and even economic consequences. Both prokaryotes and eukaryotes inhabiting such environments carry cellular systems that maintain the metal homeostasis. The ciliate protists tolerate elevated concentrations of metals, which are accumulated, bound to metallothioneins (MTs) peculiar to these organisms. Copper is one of such...
August 2011
Regression model for the study of sole and cumulative effect of temperature and solar radiation on wheat yield
The effect of variability in temperature, solar radiation and photothermal quotient were studied under varying planting windows in three wheat genotypes to cope environmental vulnerability. Regression models are regarded as valuable tools forthe evaluation of temperature, solar radiation and photothermal quotient effects on wheat yield to bring its resilience to climatic vulnerability. The objective of this study...
August 2011
Cloning, recombinant expression and characterization of a new glucoamylase gene from Aureobasidium pullulans NRRL 12974 and its potential application in raw potato starch degradation
A new amylase gene APGA1 was cloned from Aureobasidium pullulans NRRL 12974 and expressed in Pichia pastoris. This is the first report on cloning and expression of amylolytic gene from the industrially important microorganism A. pullulans. The purified recombinant protein with MW of 66 kDa and specific activity of 298.02 Umg-1 protein was veriï¬ed as a glucoamylase by its hydrolytic mode. This...
August 2011
Evaluation of xylanases from Aspergillus niger and Trichoderma sp. on dough rheological properties
Although starch is the main polysaccharide used in the fermentation of bread dough, wheat flour also contain some non-starch polysaccharides such as pentosans or hemicelluloses, which may contribute up to 3% of the total polysaccharide content of the flour. Despite being present in relatively low amounts, pentosans and hemicelluloses play an important role in dough rheology and bread properties. The aim of this work is...
August 2011
Photobleaching of Sn(IV) chlorine e6 dichloride trisodium salt in different environments
The photobleaching of Sn(IV) chlorine e6 dichloride trisodiuim salt in variety of solvents was investigated. The absorption of photosensitizer in polar solvent was much higher than in non-polar solvent. The results prove that this photosensitizerwas high water soluble. The photosensitizer absorption decreased at all bands for all solvents. Increasing the irradiation time leads to gradual decrease in the...
August 2011
Study of safflower on blood lactate concentration and exercise function of mice after exercise
Effects of safflower extracts on anti-fatigue and exercise function of mice were studied in this paper. The blood lactate concentration (BLA) experiment results indicated that the BLA of mice in the experimental groups were significantly lower than that in the control groups (p < 0.05) after swimming and rolling stick exercise. The exercise function of mice was evaluated by a calculation of the rolling-stick time,...
August 2011
Pharmaceutically important plants used in traditional system of Arab medicine for the treatment of livestock ailments in the kingdom of Saudi Arabia
A study on the pharmaceutical importance of some medicinal pants used for the treatment of livestock ailments was conducted during summer 2010 in different parts of Saudi Arabia, where no such study has been conducted so far. The aim of the study was to identify and document the medicinal plants used for the treatment of different livestock ailment in the traditional system of Arab medicine....
August 2011
In vitro protective effect of Pandanus ordoratissimus extract on ultraviolet B (UVB)-induced DNA damage
Pandanus ordoratissimus is an herb which has been widely used in Thai traditional medicines. A number of studies provided experimental evidence indicating that extracts from roots and leaves of P. ordoratissimus had powerful antioxidant and anti-inflammatory activities. However, the protective effects of extract from P. ordoratissimus flowers on ultraviolet B (UVB)-induced DNA damage have...
August 2011
Indication of bioactive candidates among body volatiles of gregarious adult locusts Locusta migratoria manilensis by electroantennography (EAG) test
Gregarious adult locusts are believed to release many bioactive volatiles from their bodies for the mediation of their biological characteristics. The determination of these bioactive body volatiles can contribute to the development of new, environmentally benign methods of locust control. An important locust, Locusta migratoria manilensis is an extremely destructive agricultural pest in...
August 2011
Effect of collection techniques on cumulus oocyte complexes (COCs) recovery, in vitro maturation and fertilization of goat oocytes
The experiment was undertaken to study the effect of collection techniques on cumulus oocyte complexes (COCs) recovery, in vitro maturation (IVM) and in vitrofertilization (IVF) of goat oocytes. COCs were collected by three techniques viz. puncture, slicing and aspiration of goat ovaries obtained at slaughterhouse. It was observed that, the total number of COCs/ovary as well...
August 2011
Lignocellulose degradation, enzyme production and protein enrichment by Trametes versicolor during solid-state fermentation of corn stover
Microbial conversion of corn stover by white rot fungi has the potential to increase its ligninolysis and nutritional value, thereby transforming it into protein-enriched animal feed. Response surface methodology was applied to optimize conditions for the production of lignocellulolytic enzymes by Trametes versicolor during solid-state fermentation of corn stover, as well as enhance ligninolysis and increase...
August 2011
Anatomy of the fruit of the halophyte Crithmum maritimum L. with emphasis on the endosperm structure and histochemistry
The halophytes are plants that can survive and reproduce under high salinity. They show high potentiality as new crops plant for biosaline agriculture. Crithmum maritimum L. (Apiaceae) is one of the promising halophytes. In this paper, the endosperm structure of the fruit of this oilseeds halophyte was investigated using scanning electrons microscopy (SEM), light microscopy (LM) and fluorescence microscopy...
August 2011
The effects of dried tomato pomace and a multiple-enzyme mixture supplementation (Rovabio ExcelTM) on performance and carcass quality of broiler chickens
An experiment was conducted to study the effect of dried tomato pomace (DTP) and a multienzyme preparation supplementation (Rovabio ExcelTM) on the performance and carcass quality of broiler chickens. A 4 × 2 factorial arrangement of treatments comprising 4 dietary DTP levels (0, 8, 16 and 24%) with and without enzyme supplementation was used. Each diet was fed to 4 replicates pens of 8 broiler chicks from 1 to 42...
August 2011
Study of malondialdehyde (MDA) content, superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities in chickens infected with avian infectious bronchitis virus
This study was carried out to investigate the changes of serum oxidative index in chickens infected with avian infectious bronchitis virus. Eighty (80) 15-day-oldchickens were divided into two groups randomly: the control group and the experimental group. Birds in the experimental group were inoculated with infectious bronchitis virus, then the superoxide dismutase (SOD) and...
August 2011
In vitro antimicrobial and antioxidant activities of bark extracts of Bauhinia purpurea
Bark extracts of Bauhinia purpurea were phytochemically analyzed and evaluated for antimicrobial and antioxidant activities. The phytochemical analysis of the bark extracts revealed the presence of alkaloids, flavonoids, steroids, saponins, triterpenes and carbohydrates. While most of them were present in methanolic and aqueous extracts, one or a few of them were present in other solvent extracts. Among...
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