African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12486

Full Length Research Paper

Marker-assisted pyramiding of Xa21 and Xa7 genes conferring resistance to bacterial leaf blight in indica cultivar Bacthom7

Nguyen Thi Thu
  • Nguyen Thi Thu
  • Crop Research and Development Institute, Vietnam National University of Agriculture, Trau Qui, Gia Lam, Hanoi, Vietnam.
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Vu Hong Quang
  • Vu Hong Quang
  • Crop Research and Development Institute, Vietnam National University of Agriculture, Trau Qui, Gia Lam, Hanoi, Vietnam.
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Mai Van Tan
  • Mai Van Tan
  • Crop Research and Development Institute, Vietnam National University of Agriculture, Trau Qui, Gia Lam, Hanoi, Vietnam.
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Vu Đuc Lam
  • Vu Đuc Lam
  • Crop Research and Development Institute, Vietnam National University of Agriculture, Trau Qui, Gia Lam, Hanoi, Vietnam.
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Nakano Toshitsugu
  • Nakano Toshitsugu
  • Crop Research and Development Institute, Vietnam National University of Agriculture, Trau Qui, Gia Lam, Hanoi, Vietnam.
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Nguyen Thi Hue
  • Nguyen Thi Hue
  • Crop Research and Development Institute, Vietnam National University of Agriculture, Trau Qui, Gia Lam, Hanoi, Vietnam.
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Nong Thi Hue
  • Nong Thi Hue
  • Faculty of Biotechnology, Vietnam National University of Agriculture, Trau Qui, Gia Lam, Hanoi, Vietnam.
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Nguyen Viet Long
  • Nguyen Viet Long
  • Faculty of Biotechnology, Vietnam National University of Agriculture, Trau Qui, Gia Lam, Hanoi, Vietnam.
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Nguyen Viet Long
  • Nguyen Viet Long
  • Faculty of Agronomy, Vietnam National University of Agriculture, Trau Qui, Gia Lam, Hanoi, Vietnam.
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Nguyen Van Hoan
  • Nguyen Van Hoan
  • Crop Research and Development Institute, Vietnam National University of Agriculture, Trau Qui, Gia Lam, Hanoi, Vietnam.
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Vu Van Liet
  • Vu Van Liet
  • Crop Research and Development Institute, Vietnam National University of Agriculture, Trau Qui, Gia Lam, Hanoi, Vietnam.
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Nguyen Thi Mien
  • Nguyen Thi Mien
  • Center for Inbred Rice Breeding, Field Crop Research Institute, Lien Hong, Gia Loc, Hai Duong, Vietnam.
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Tran Thi Lien
  • Tran Thi Lien
  • Center for Inbred Rice Breeding, Field Crop Research Institute, Lien Hong, Gia Loc, Hai Duong, Vietnam.
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Nguyen Dinh Trung
  • Nguyen Dinh Trung
  • Faculty of Land Management, Vietnam National University of Agriculture, Trau Qui, Gia Lam, Hanoi, Vietnam.
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Tran Thi Thu Hoai
  • Tran Thi Thu Hoai
  • Plant Resources Center, Vietnamese Academy of Agricultural Science, Ankhanh, Hoaiduc, Hanoi, Vietnam.
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  •  Received: 04 May 2017
  •  Accepted: 14 June 2017
  •  Published: 12 December 2018

Abstract

Bacterial leaf blight (BLB) of rice is one of the most destructive diseases affecting rice fields. Xanthomonas oryzae pv. oryzae (Xoo) is the causal agent of BLB. Two BLB resistance genes, Xa21 and Xa7, were transferred into the susceptible indica cultivar Bacthom7 (BT7) by using marker-assisted selection with markers pTA248 for Xa21 and ID7 for Xa7. Improved BT7 lines carrying the two resistance genes were inoculated with three isolates of the Xoo from Northern Vietnam and evaluated for agronomic traits. Artificial inoculation of 13 lines with three Xoo races identified nine highly resistant lines with wide-spectrum resistance to Xoo, including D1, D2, D3, D6, D7, D8, D9, D10 and D12. These lines were similar to recurrent parent BT7 with regard to external appearance, yield performance and grain quality. On the basis of agronomic traits and the level of resistance to BLB, two promising lines, D6 and D9 were further selected. These two lines could efficiently contribute to rice production for food security and food safety in northern Vietnam.

 

Key words: Xanthomonas oryzae pv. oryzae, resistance genes, near-isogenic lines, marker-assisted selection (MAS), improved rice lines. 

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This article is published under the terms of the Creative Commons Attribution License 4.0

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