African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12482

Full Length Research Paper

Research on human placenta-derived mesenchymal stem cells transfected with pIRES2-EGFP-VEGF165 using liposome

Liu Zhihui1,2, Meng Guangwei2, Wang Bowei3*, Liu Chunli4, Zhou Yanmin5 and Wang Yue6
1Institute of Mechanical Science and Engineering, Jilin University, Changchun 130021, Jilin, China. 2Prosthodontics Department of Stomatological Hospital, Jilin University, Changchun 130021, Jilin, China. 3Obstetrics and Gynecology Department of the Second Hospital, Jilin University, Changchun 130021, Jilin, China. 4Anesthesiology Department of Stomatological Hospital, Jilin University, Changchun 130021, Jilin, China. 5Implantology Department of Stomatological Hospital, Jilin University, Changchun 130021, Jilin, China. 6Oral and Maxillofacial Surgery Department of Stomatological Hospital, Jilin University, Changchun 130021, Jilin, China.
Email: [email protected]

  •  Accepted: 14 March 2011
  •  Published: 01 August 2011

Abstract

The experiment adopting reverse transcription polymerase chain reaction (RT-PCR) technology, amplified hVEGF165 gene fragments from human leukemia cells HL-60. hVEGF165 gene was reconstructed in pIRES2-EGFP and transferred into the human placenta-derived mesenchymal stem cells (HPMSCs) by liposome-mediated method successfully. The mRNA and protein of hVEGF165 in the transferred cells was detected by RT-PCR and Western blot, and the results showed that hVEGF165mRNA and the protein expressed by HPMSCs transfected with pIRES2-EGFP-hVEGF165 was significantly more than HPMSCs transfected with pIRES2-EGFP. EGFP expression was observed under fluorescence microscope, which proved that the report gene was successfully transferred to the target cells. hVEGF biology activity and cell proliferation activity of HPMSCStransfected with pIRES2-EGFP-hVEGF165 was detected by MTT array, which showed that hVEGF165 can promote the proliferation of HPMSCS; however, hVEGF165 biology activity of HPMSCS transfected with pIRES2-EGFP-hVEGF165 was significantly more than HPMSCs transfected with pIRES2-EGFP. Identification of multipotentiality showed that HPMSCS transfected with pIRES2-EGFP-VEGF165 still maintained multipotentiality.

 

Key words: Transfect, human placenta-derived mesenchymal stem cells, hVEGF165.

Abbreviation

VEGF, Vascular endothelial growth factor; BMSCS, bone marrow mesenchymal stem cells; ADSCs, adipose-derived stem cells; HPMSCs, human placenta-derived mesenchymal stem cells; RT-PCR, reverse transcription polymerase chain reaction; DMSO, dimethylsulfoxide.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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