African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12486

Article in Press

Antifungal effect of cow urine extracts against Colletotrichum capsici causing leaf spot disease of turmeric

R.S. MISHRA

  •  Received: 13 February 2015
  •  Accepted: 12 June 2015
Turmeric is an herbaceous perennial crop which is native of the genus Curcuma longa L., belonging to family Zingiberaceae. It has versatile uses in flavouring, dye making, drug preparation, cosmetics and medicine. The turmeric plant highly prone to several fungal diseases, among them leaf spot disease of turmeric resulting to huge losses of 25.83 to 62.12% fresh weight and 42.10 to 62.10% dry weight of rhizome.The five plant extract namely; neem (Azadirachta indica), Onion (Allium cepa); Garlic (Allium sativum), madar (Calotropis procera) and datura (Datura stramonium) were used with Cow urine, acetone and distilled water separately and all extract mixed with botanical formulations against Colletotrichum capsici..All the nineteen treatments showed significant reduction of mycelial growth of the test fungus. The maximum reduction was found in cow urine based botanical formulation 50.83-56.05 % followed by acetone based botanical formulation 49.92-54.31 %, distilled water based botanical formulation 44.51-52.35 %. The effectiveness of the individual cow urine based onion extract was (51.58%) at 20% concentration after 72hrs incubation followed by neem extract (48.63%) at 20% concentration after 96hrs of incubation, datura extract (46.58%) at 20% concentration after 72hrs incubation. Acetone based plant extract was also found effective for mycelial growth inhibition of Colletotrichum capsici. The highest inhibition was recorded in onion extract (49.21%) followed by neem extract (45.79%) at 20% concentration after 72hrs incubation, garlic extract (42.94%) at 20% concentration after 96hrs of incubation. Distilled water based botanical formulation were found least effective except 120 hrs of incubation at 20% concentration. Leaf spot of turmeric was significantly minimized by all treatment over the control. Cow urine based botanical formulation was found most effective in comparison to acetone based botanical formulation and distilled water based botanical formulation at 60, 75 and 90 days after planting, The end of fourth spray at 120 days after planting the percent disease control was not significant among the botanical formulation and was significant with control.

Keywords: C.capsici, cow urine, plant extracts, mycelial growth, control, leaf spot,