African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12486

Article in Press

In vitro propagation of the wild medicinal plant, caper (Capparis spinosa L.)

Adel M. Elmaghrabi*, Elmundr Abughnia and Salem Hamoud

  •  Received: 26 October 2016
  •  Accepted: 30 March 2017
Micropropagation of caper (Capparis spinosa L.) was done using wild leaves from Libya. High percentage (95%) of callus induction was obtained from Murashige and Skoog (MS) medium supplemented with 1.2 mg l-1 2,4-D + maltose. The effect of various concentrations (0.0, 0.5. 1.0 and 2.0 mg l-1) of cytokines (BAP, 6-γ-γ-[Dimethylallylamino]-purine 2ip, Kin and Zea) supplemented with MS medium was evaluated for callus development in vitro. MS medium with 2 mg l-1 BAP was found to be the best concentration and hormone for the establishment of callus shooting and rooting. Multiple branches and leaves were obtained from MS medium supplemented with 2.0 mg l-1 6-γ-γ-[Dimethylallylamino]-purine (2ip), kin or Zea. High frequency (82%) of rooting was obtained from MS medium supplemented with 2 mg l-1 BAP. Regenerated plantlets were successfully acclimatized in green house.

Keywords: Capers, in vitro propagation, callus.

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