Tomato (Solanum lycopersicum L.) is one of the most widely consumed vegetables in the World. This study evaluated the proximate, phytochemical and microbial composition on two local and two improved accessions of tomato. The two local accessions (BNL and EKL) were obtained from traditional agriculture system in Udo community Edo State while the improved accessions (IMPA and IMPB) were obtained from Nigerian Institute of Horticultural Research, Ibadan. The accessions were tried in the experimental farm University of Benin. Standard methods were used for phytochemical and proximate analyses while serial dilution method was used for microbial analysis. Variations were observed among the four accessions for phytochemicals and proximate value. Aspergillus sp, Penicillium sp, Saccharomyces sp, Pestalucia sp and Mucor sp were associated with the ripe and unripe fruits of the accessions. The study indicates that the two local accessions are phytochemically comparable to the exotic ones

Keywords: Tomato (Solanum lycopersicum), Traditional agriculture, Phytochemical, Food security, Microbes