Tri-Phala residue (TR) was studied as a substrate for isoquercitrin and gallic acid productions by solid-state fermentation of A. niger in the packed bed bioreactor aerated at of 0.1, 0.3 and 0.5 vvm. The contents of biomass, isoquercitrin and gallic acid in the fermented TR along the height of the bioreactor were analyzed. The highest fungal growth rate was found on middle position in the SSF with 0.5 vvm aeration as 1.96±0.02 h-1. The highest isoquercitrin production was found in 0.1 vvm aerate condition at top position of PBB at 48 h as 67.97±2.55 mg g-1 or 1.41±0.06 h-1 production rate, while the highest gallic acid one was found in the SSF with 0.3 vvm aeration at middle position at 60 h as 187.23±11.05 mg g-1 with 3.12±0.09 h-1 production rate. Insufficient and excessive aeration had a negative effect on biomass, isoquercitrin and gallic productions as they decreased on the 0.5 vvm aerating condition.

Keywords: Isoquercitrin, gallic acid, Aspergillus niger, solid state, fermentation, tri-phala