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References
|
Agyeman K, Berchie JN, Osei-Bonsu I, Fordjour JK (2015). Seed yield and agronomic performance of seven improved cowpea (Vigna unguiculata L.) varieties in Ghana. African Journal of Agricultural Research 10(4):215-221. |
|
|
Alemu M, Asfaw Z, Woldu Z, Fenta BA, Medvecky B (2016). Cowpea (Vigna unguiculata (L.) Walp.) (Fabaceae) landrace diversity in northern Ethiopia. International Journal of Biodiversity and Conservation 8(11):297-309. |
|
|
Amorim LLB, Ferreira-Neto JRC, Bezerra-Neto JP, Pandolfi V, de Araújo FT, da Silva Matos MK, Benko-Iseppon AM (2018). Cowpea and abiotic stresses: identification of reference genes for transcriptional profiling by qPCR. Plant Methods 14:1-17. |
|
|
Andersen C, Jensen J, Orntoft T (2004). Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Cancer Research 64(15):5245-5250. |
|
|
Appiah F, Asibuo JY, Kumah P (2011). Physicochemical and functional properties of bean flours of three cowpea (Vigna unguiculate L. Walp) varieties in Ghana. African Journal of Food Science 5(2):100-104. |
|
|
Artico S, Nardeli SM, Brilhante O, Grossi-de-Sa MF, Alves-Ferreira M (2010). Identification and evaluation of new reference genes in Gossypium hirsutum for accurate normalization of real-time quantitative RT-PCR data. BMC Plant Biology 10:1-12. |
|
|
Auler PA, Benitez LC, Amaral MN, Vighi IL, Dos-Santos-Rodrigues G, da Maia LC, Braga EJ (2017). Evaluation of stability and validation of reference genes for RT-qPCR expression studies in rice plants under water deficit. Journal of Applied Genetics 58:163-177. |
|
|
Beshir B, Amsalu B, Dagmawit T, Selamawit K, Teamir M, Bezawit Y (2019). Cowpea production, marketing and utilization in Ethiopia. Ethiopian Institute of Agricultural Research 121p. |
|
|
Borges A, Tsai SM, Caldas DG (2012). Validation of reference genes for RT-qPCR normalization in common bean during biotic and abiotic stresses. Plant Cell Reports 31:827-838. |
|
|
Bustin SA, Benes V, Garson J, Hellemans J, Huggett J, Kubista M, Mueller R, Nolan T, Pfaffl MW, Shipley G, Wittwer CT (2013). The need for transparency and good practices in the qPCR literature. Nature Methods 10(11):1063-1067. |
|
|
Bustin SA, Benes V, Garson JA, Hellemans J, Huggett J, Kubista M, Wittwer CT (2009). The MIQE Guidelines: M inimum Information for Publication of Q uantitative Real-Time PCR Experiments. |
|
|
Caldana C, Scheible WR, Mueller-Roeber B, Ruzicic SA (2007). A quantitative RT-PCR platform for high-throughput expression profiling of 2500 rice transcription factors. Plant Methods 3:1-9. |
|
|
Chen K, Fessehaie A, Arora R (2012). Dehydrin metabolism is altered during seed osmopriming and subsequent germination under chilling and desiccation in Spinacia oleracea L. cv. Bloomsdale: Possible role in stress tolerance. Plant Science 183:27-36. |
|
|
Chinma CE, Emelife IG, Alemede IC (2008). Physicochemical and functional properties of some Nigerian cowpea varieties. Pakistan Journal of Nutrition 7(1):186-190. |
|
|
Choudhary T (2019). 14 Best benefits of cowpeas (Lobia) for skin, hair and health. Available at: View |
|
|
Czechowski T, Stitt M, Altmann T, Udvardi MK, Scheible WR (2005). Genome-wide identification and testing of superior reference genes for transcript normalization in Arabidopsis. Plant Physiology 139(1):5-17. |
|
|
Da Silva HAP, Nardeli SM, Alves?Ferreira M, Simões?Araújo JL (2015). Evaluation of Reference Genes for RT?qPCR Normalization in Cowpea under Drought Stress during Biological Nitrogen Fixation. Crop Science 55(4):1660-1672. |
|
|
Fang Y, Hirsch AM (1998). Studying early nodulin gene ENOD40 expression and induction by nodulation factor and cytokinin in transgenic alfalfa. Plant Physiology 116(1):53-68. |
|
|
Gao M, Liu Y, Ma X, Shuai Q, Gai J, Li Y (2017). Evaluation of reference genes for normalization of gene expression using quantitative RT-PCR under aluminum, cadmium, and heat stresses in soybean. PLoS One 12(1):e0168965. |
|
|
Gao ZH, Wei JH, Yang Y, Zhang Z, Zhao WT (2012). Selection and validation of reference genes for studying stress-related agarwood formation of Aquilaria sinensis. Plant Cell Reports 31:1759-1768. |
|
|
Gavazzi F, Pigna G, Braglia L, Gianì S, Breviario D, Morello L (2017). Evolutionary characterization and transcript profiling of β-tubulin genes in flax (Linum usitatissimum L.) during plant development. BMC Plant Biology 17:1-16. |
|
|
Hamza TA, Alebejo AL (2017). Isolation and characterization of rhizobia from rhizosphere and root nodule of Cowpea, elephant and lab plants. International Journal of Novel Research in Interdisciplinary Studies 4:1-7. |
|
|
Horn L, Nghituwamata SN, Ueitele I (2022). Cowpea production challenges and contribution to livelihood in Sub-Sahara region. Agricultural Sciences 13(01):25-32. |
|
|
Kozera B, Rapacz M (2013). Reference genes in real-time PCR. Journal of Applied Genetics 54:391-406. |
|
|
Kyei-Boahen S, Savala CE, Chikoye D, Abaidoo R (2017). Growth and yield responses of cowpea to inoculation and phosphorus fertilization in different environments. Frontiers in Plant Science 8:238588. |
|
|
Langyintuo AS, Lowenberg-DeBoer J, Faye M, Lambert D, Ibro G, Moussa B, Kushwaha A, Musa S, Ntoukam G (2003). Cowpea supply and demand in West and Central Africa. Field Crops Research 82(2-3):215-231. |
|
|
Li R, Cui K, Xie Q, Xie S, Chen X, Zhuo L, Cao A, Shen H, Jin X, Wang F, Li H (2021). Selection of the reference genes for quantitative gene expression by RT-qPCR in the desert plant Stipagrostis pennata. Scientific Reports 11(1):21711. |
|
|
Livak KJ, Schmittgen TD (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2− ΔΔCT method. Methods 25(4):402-408. |
|
|
Lü J, Yang C, Zhang Y, Pan H (2018). Selection of reference genes for the normalization of RT-qPCR data in gene expression studies in insects: a systematic review. Frontiers in Physiology 9:1560 |
|
|
Ma H, Bell KN, Loker RN (2021). qPCR and qRT-PCR analysis: Regulatory points to consider when conducting biodistribution and vector shedding studies. Molecular Therapy-Methods and Clinical Development 20:152-168. |
|
|
Makanjuola DO, Labbo Z, Sidi D, Egbulefu CS, Suleiman RO (2023). Influence of two Glomus species on root nodule number of cowpea (Vigna unguiculata (L) walp) varieties on Alectra vogelii inoculated soil. Science World Journal 18(1):90-96. |
|
|
Mao M, Xue Y, He Y, Zhou X, Hu H, Liu J, Feng L, Yang W, Luo J, Zhang H, Li X, Ma J (2021). Validation of Reference Genes for Quantitative Real-Time PCR Normalization in Ananas comosus var. bracteatus During Chimeric Leaf Development and Response to Hormone Stimuli. Frontiers in Genetics 12:716137. |
|
|
MOFA (2019). Catalogue of Crop Varieties Released and Registered in Ghana. Retrieved from View |
|
|
Morgante CV, Guimarães PM, Martins AC, Araújo AC, Leal-Bertioli SC, Bertioli DJ (2011). Reference genes for quantitative reverse transcription-polymerase chain reaction expression studies in wild and cultivated peanut. BMC Research Notes 4:1-11. |
|
|
Nkomo G (2020). Genetic Diversity and Screening of Cowpea (Vigna unguiculata L. Walp.) Genotypes for Drought Tolerance in South Africa (Doctoral dissertation, Central University of Technology, Free State). http://ir.cut.ac.za/handle/11462/2309 |
|
|
Nyaga JW, Njeru EM (2020). Potential of Native Rhizobia to Improve Cowpea Growth and Production in Semiarid Regions of Kenya. Frontiers in Agronomy 2:606293. |
|
|
Oruru MB, Njeru EM, Pasquet R, Runo S (2018). Response of a wild-type and modern cowpea cultivars to arbuscular mycorrhizal inoculation in sterilized and non-sterilized soil. Journal of Plant Nutrition 41(1):90-101. |
|
|
Pfaffl MW (2007). Relative quantification. In Real-time PCR (pp. 89-108). Taylor & Francis. |
|
|
Pfaffl MW, Tichopad A, Prgomet C, Neuvians TP (2004). Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper-Excel-based tool using pair-wise correlations. Biotechnology Letters 26:509-515. |
|
|
Qi S, Yang L, Wen X, Hong Y, Song X, Zhang M, Dai S (2016). Reference gene selection for RT-qPCR analysis of flower development in Chrysanthemum morifolium and Chrysanthemum lavandulifolium. Frontiers in Plant Science 7:173132. |
|
|
Rodrigues TB, Dhandapani RK, Duan JJ, Palli SR (2017). RNA interference in the Asian longhorned beetle: identification of key RNAi genes and reference genes for RT-qPCR. Scientific Reports 7(1):8913. |
|
|
Sánchez-Navarro V, Zornoza R, Faz Á, Fernández JA (2019a). Comparing legumes for use in multiple cropping to enhance soil organic carbon, soil fertility, aggregates stability and vegetables yields under semi-arid conditions. Scientia Horticulturae 246:835-841. |
|
|
Sánchez-Navarro V, Zornoza R, Faz Á, Fernández JA (2019b). Does the use of cowpea in rotation with a vegetable crop improve soil quality and crop yield and quality? A field study in SE Spain. European Journal of Agronomy 107:10-17. |
|
|
Sani A, Abubakar BZ, Yakubu DH, Atala TK, Abubakar L (2014). Socio-economic factors influencing adoption of dual-purpose cowpea production technologies in Bichi Local Government Area of Kano State, Nigeria. Asian Journal of Agricultural Extension, Economics and Sociology 3(4):257-274. |
|
|
Silver N, Best S, Jiang J, Thein SL (2006). Selection of housekeeping genes for gene expression studies in human reticulocytes using real-time PCR. BMC Molecular Biology 7:1-9. |
|
|
Summerfield RJ, Huxley PA, Steele W (1974). Cowpea (Vigna unguiculata L. Walp). Field Crops Research 53(1-3):187-204. |
|
|
Sun H, Jiang X, Sun M, Cong H, Qiao F (2019). Evaluation of reference genes for normalizing RT-qPCR in leaves and suspension cells of Cephalotaxus hainanensis under various stimuli. Plant Methods 15:1-11. |
|
|
Tang X, Zhang N, Si H, Calderón-Urrea A (2017). Selection and validation of reference genes for RT-qPCR analysis in potato under abiotic stress. Plant Methods 13:1-8. |
|
|
Timko MP, Singh BB (2008). Cowpea, a multifunctional legume. In 'Genomics of tropical crop plants. Ch. 10. (Eds PH Moore, R Ming) pp. 227-258. |
|
|
Udvardi MK, Czechowski T, Scheible WR (2008). Eleven golden rules of quantitative RT-PCR. The Plant Cell 20(7):1736-1737. |
|
|
Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, Speleman F (2002). Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biology 3:1-12. |
|
|
Wan Q, Chen S, Shan Z, Yang Z, Chen L, Zhang C, Zhou X (2017). Stability evaluation of reference genes for gene expression analysis by RT-qPCR in soybean under different conditions. PLoS ONE 12(12):e0189405. |
|
|
Xie F, Xiao P, Chen D, Xu L, Zhang B (2012). miRDeepFinder: a miRNA analysis tool for deep sequencing of plant small RNAs. Plant Molecular Biology 80:75-84. |
|
|
Xie H, Li B, Chang Y, Hou X, Zhang Y, Guo S, Miao Y, Wang Q, Chen S, Su Y, Li Y, Dai S (2021). Selection and Validation of Reference Genes for RT-qPCR Analysis in Spinacia oleracea under Abiotic Stress. BioMed Research International 3:4853632. |
|
|
Xue W, Wang L, Li X, Tang M, Li J, Ding B, Kawabata S, Li Y, Zhang Y (2022). Evaluation of Reference Genes for Quantitative PCR in Eustoma grandiflorum under Different Experimental Conditions. Horticulturae 8:164. |
|
|
Yang Y, Zhang X, Chen Y, Guo J, Ling H, Gao S, Xu L (2016). Selection of reference genes for normalization of microRNA expression by RT-qPCR in sugarcane buds under cold stress. Frontiers in Plant Science 7:86. |
|
|
Yu Y, Zhang G, Chen Y, Bai Q, Gao C, Zeng L, Li Z, Cheng Y, Chen J, Sun X, Guo L (2019). Selection of reference genes for qPCR analyses of gene expression in ramie leaves and roots across eleven abiotic/biotic treatments. Scientific Reports 9(1):20004. |
|
|
Zhang Y, Chen J, Chen G, Ma C, Chen H, Gao X, Tian Z, Cui S, Tian Z, Guo J, Wan F, Zhou Z (2020). Identification and Validation of Reference Genes for Quantitative Gene Expression Analysis in Ophraella communa. Frontiers in Physiology 11:355. |
|
|
Zheng T, Chen Z, Ju Y, Zhang H, Cai M, Pan H, Zhang Q (2018). Reference gene selection for qRT-PCR analysis of flower development in Lagerstroemia indica and L. speciosa. PLoS One 13(3):e0195004. |
|
|
Zhu J, Zhang L, Li W, Han S, Yang W, Qi L (2013). Reference gene selection for quantitative Real-Time PCR normalization in Caragana intermedia under different abiotic stress conditions. PloS One 8(1):e53196. |
|
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