African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12486

Full Length Research Paper

DNA isolation and optimization of PCR protocol for ISSR analysis of Girardinia diversifolia: A medicinal and economic plant species from Nepal Himalaya

Bijay Raj Subedee
  • Bijay Raj Subedee
  • Research Centre for Applied Science and Technology, Tribhuvan University, Kirtipur, Kathmandu, Nepal.
  • Google Scholar
Giri Raj Tripathi
  • Giri Raj Tripathi
  • Central Department of Biotechnology, Tribhuvan University, Kirtipur, Kathmandu, Nepal.
  • Google Scholar
Ram Prasad Chaudhary
  • Ram Prasad Chaudhary
  • Research Centre for Applied Science and Technology, Tribhuvan University, Kirtipur, Kathmandu, Nepal.
  • Google Scholar


  •  Received: 20 August 2020
  •  Accepted: 09 October 2020
  •  Published: 31 October 2020

References

Aboul-Maaty NF, Oraby HAS (2019). Extraction of high-quality genomic DNA from different plant orders applying a modified CTAB-based method. Bulletin of the National Research Centre 43(1):25.
Crossref

 

Amom T, Tikendra L, Rahaman, Nongdam P (2018). Evaluation of genetic relationship between 15 bamboo species of North-East India based on ISSR marker analysis. Molecular Biology Research Communications 7(1):7-15.

 
 

Anerao J, Jha V, Desai N (2016). Optimization of DNA Extraction Methods from Garcinia species for ISSR-PCR, RAPD-PCR and DNA Barcoding. Asian Journal of Biotechnology 9(1):35-42.
Crossref

 
 

Anne C (2006). Choosing the right molecular genetic markers for studying biodiversity: From molecular evolution to practical aspects. Genetica 127:101-120.
Crossref

 
 

Arruda SR, Pereira DG, Silva-Castro MM, Brito MG, Waldschmidt AM (2017). An optimized protocol for DNA extraction in plants with a high content of secondary metabolites, based on leaves of Mimosa tenuiflora (Willd.) Poir. (Leguminosae). Genetics and Molecular Research 16(3):1-9.
Crossref

 
 

Barakoti TP, Shrestha KP (2008). Commercial utilization of Allo (Girardinia diversifolia) by the Rais of Sankhuwasabha for income generation. Banko Janakari 18(1):18-24.
Crossref

 
 

Bharmauria V, Narang N, Verma V, Sharma S (2009). Genetic variation and polymorphism in the Himalayan nettle plant Urtica dioica based on RAPD marker. Journal of Medicinal Plants Research 3(3):166-170.

 
 

Choudhary K, Mathur N, Choudhary OP, Pillai U (2008). Protocol for Isolation of Genomic Dna from Dry and Fresh Leaves of Vigna Species Suitable for Rapd and Restriction Digestion. Advances in Biological Research 2(5-6):83-89.

 
 

Devi AA, Brajendra N, Dinachandra M (2018). Genetic Diversity Analysis in Chilli (Capsicum annuum L.) Found in Manipur Using RAPD Markers. International Journal of Current Microbiology and Applied Sciences 7(10):257-262.
Crossref

 
 

Doyle JJ, Doyle JL (1987). A Rapid DNA Isolation Procedure for Small Quantities of Fresh Leaf Tissue. Phytochemical Bulletin 19:11-15.

 
 

Fernie AR, Klee HJ (2011). The Use of Natural Genetic Diversity in the Understanding of Metabolic Organization and Regulation. Frontiers in Plant Science 2:59.
Crossref

 
 

Friar EA (2005). Isolation of DNA from Plants with Large Amounts of Secondary Metabolites. In Methods in Enzymology 395:1-12.
Crossref

 
 

Friis I (1981). A Synopsis of Girardinia (Urticaceae). Kew Bulletin 36(1):143-157.
Crossref

 
 

John ME (1992). An efficient method for isolation of RNA and DNA from plants containing polyphenolics. Nucleic Acids Research 20(9):2381-2381.
Crossref

 
 

Khanuja SP, Shasany AK, Darokar MP, Kumar S (1999). Rapid isolation of DNA from dry and fresh samples of plants producing large amounts of secondary metabolites and essential oils. Plant Molecular biology Reporter 17(1):74.
Crossref

 
 

Kit YS, Chandran S (2010). A simple, rapid and efficient method of isolating DNA from Chokanan mango ( Mangifera indica L.). African Journal of Biotechnology 9(36):5805-5808.

 
 

Kumar A, Singh I, Badari NS (2018). Extraction of Genomic DNA from Dried mature leaves of Eclipta alba (L.) suitable for ISSR-PCR and other DNA fingerprinting and Barcoding techniques. International Journal of Molecular Biotechnology 4(2):23-30.

 
 

Kumari M, Jadhav AS, Chahande RV (2020). Isolation of genomic DNA from groundnut plant by modified, rapid and efficient protocol. Journal of Pharmacognosy and Phytochemistry 9(1):2268-2271.

 
 

Mohamad A, Alhasnawi AN, Kadhimi AA, Isahak A, Wan Yusoff WM, Che Radziah CMZ (2017). DNA Isolation and Optimization of ISSR-PCR Reaction System in Oryza sativa L. International Journal on Advanced Science, Engineering and Information Technology 7(6):2264.
Crossref

 
 

Mujeeb F, Bajpai P, Pathak N, Verma SR (2017). Genetic Diversity Analysis of Medicinally Important Horticultural Crop Aegle marmelos by ISSR Markers. In L. Domingues (Ed.), PCR. Springer New York. pp. 195-211. 
Crossref

 
 

Nilkanta H, Amom T, Tikendra L, Rahaman H, Nongdam P (2017). ISSR Marker Based Population Genetic Study of Melocanna baccifera (Roxb.) Kurz: A Commercially Important Bamboo of Manipur, North-East India. Scientifica 2017:1-9.
Crossref

 
 

Njogu PM, Thoithi GN, Mwangi JW, Kamau FN, Kibwage IO, Kariuki ST, Mwalukumbi JM (2011). Phytochemical and Antimicrobial Investigation of Girardinia diversifolia (Link)Friis (Urticaceae). East and Central African Journal of Pharmaceutical Sciences 14(3):89-94.

 
 

Osena G, Nyaboga E, Amugune N (2017). Rapid and Efficient Isolation of High Quality DNA from Cassava (Manihot esculenta Crantz) Suitable for PCR Based Downstream Applications. Annual Research and Review in Biology 12:1-10.
Crossref

 
 

Polunin O, Stainton A (1984). Flowers of the Himalaya. Oxford University Press.

 
 

Porebski S, Bailey LG, Baum BR (1997). Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components. Plant Molecular Biology Reporter 15(1):8-15.
Crossref

 
 

Puchooa D (2004). A simple, rapid and efficient method for the extraction of genomic DNA from lychee (Litchi chinensis Sonn.). African Journal of Biotechnology 3(4):253-255.
Crossref

 
 

Reddy MP, Sarla N, Siddiq EA (2002). Inter simple sequence repeat (ISSR) polymorphism and its application in plant breeding. Euphytica 128(1):9-17.
Crossref

 
 

Sá O, Pereira JA, Baptista P (2011). Optimization of DNA Extraction for RAPD and ISSR Analysis of Arbutus unedo L. Leaves. International Journal of Molecular Sciences 12:4156-4164.
Crossref

 
 

Sarrazola JH, Alzate FA (2019). Obtaining DNA from Urticaceae: Overcoming the challenges associated with chemical compounds and herbarium specimens. International Journal of Molecular Biology 4(5):158-165.
Crossref

 
 

Shrestha SS, Sut S, Ferrarese I, Barbon Di Marco S, Zengin G, De Franco M, Pant DR, Mahomoodally MF, Ferri N, Biancorosso N, Maggi F, Dall Acqua S, Rajbhandary S (2020). Himalayan Nettle Girardinia diversifolia as a Candidate Ingredient for Pharmaceutical and Nutraceutical Applications-Phytochemical Analysis and In Vitro Bioassays. Molecules 25:1563.
Crossref

 
 

Shrestha R (1997). Cytological Studies in Girardinia Diversifolia (Link) Friis. Pakistan Journal of Botany 29(2):263-269.

 
 

Shukla R, Sharma DC, Pathak N, Bajpai P (2018). Genomic DNA Isolation from High Polyphenolic Content Grewia asiatica L. Leaf Without Using Liquid Nitrogen. Iranian Journal of Science and Technology, Transactions A: Science 42:347-351.
Crossref

 
 

Singh SC, Shrestha R (1988). Girardinia diversifolia (Urticaceae), a non-conventional fiber resource in Nepal. Economic Botany 42:445-447.

 
 

Subedee BR, Chaudhary RP, Uprety Y, Dorji T (2020). Socio-ecological perspectives of Himalayan Giant Nettle ( Girardinia diversifolia (Link) Friis) in Nepal. Journal of Natural Fibers 17(1):9-17.
Crossref

 
 

Tiwari KL, Jadhav SK, Gupta S (2012). Modified CTAB Technique for Isolation of DNA from some Medicinal Plants. Research Journal of Medicinal Plant 6(1):65-73.
Crossref

 
 

Wu ZY, Monro AK, Milne RI, Wang H, Yi TS, Liu J, Li DZ (2013). Molecular phylogeny of the nettle family (Urticaceae) inferred from multiple loci of three genomes and extensive generic sampling. Molecular Phylogenetics and Evolution 69(3):814-827.
Crossref