African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

In vitro selective cytotoxicity of activated parasporal proteins produced by Bacillus thuringiensis serovars kumamotoensis and tohokuensis against human cancer cell lines

Maher Obeidat
  • Maher Obeidat
  • Department of Biotechnology, Faculty of Agricultural Technology, Al-Balqa Applied University, Al-Salt 19117, Jordan.
  • Google Scholar


  •  Received: 08 August 2017
  •  Accepted: 16 October 2017
  •  Published: 22 November 2017

References

Al-Momani F, Obeidat M, Saadoun I, Meqdam M (2004). Serotyping of Bacillus thuringiensis isolates, their distribution in different Jordanian habitats and pathogenicity in Drosphila melanogaster. World J. Microbiol. Biotechnol. 20:749-753.
Crossref

 

Bradford MM (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
Crossref

 
 

Carillo P, Mardarz C, Pitta-Alvarez S (1996). Isolation and selection of biosurfactant producing bacteria. World J. Microbiol. Biotechnol. 12:82-84.
Crossref

 
 

Crickmore N, Baum J, Bravo A, Lereclus D, Narva K, Sampson K, Schnepf E, Sun M, Zeigler DR (2016). Bacillus thuringiensis toxin nomenclature. 

View

 
 

Crickmore N, Zeigler DR, Feitelson J (1998). Revision of the nomenclature for the Bacillus thuringiensis pesticidal crystal proteins. Microbiol. Mol. Biol. Rev. 62:807-813.

 
 

Ekino K, Okumura S, Ishikawa T, Kitada S, Saitoh H, Akao T, Oka T, Nomura Y, Ohba M, Shin T, Mizuki E (2014). Cloning and Characterization of a Unique Cytotoxic Protein Parasporin-5 Produced by Bacillus thuringiensis A1100 Strain. Toxins (Basel). 6:1882-1895.
Crossref

 
 

Freshney RI (2005). Culture of Animal Cells, A Manual of Basic Technique, 5th edition. Hoboken NJ, John Wiley and Sons.
Crossref

 
 

Hayakawa T, Kanagawa R, Kotani Y, Kimura M, Yamagiwa M, Yamane Y, Takebe S, Sakai H (2007). Parasporin-2Ab, a newly isolated cytotoxic crystal protein from Bacillus thuringiensis. Curr. Microbiol. 55:278-283.
Crossref

 
 

Heiss P, Bernatz S, Bruchelt G, Senekowitsch-Schmidtke R (1997). Cytotoxic effect of immunoconjugate composed of glucose-oxidase coupled to an anti-ganglioside (GD2) antibody on spheroids. Anticancer Res. 17(4B):3177-3178.

 
 

Ito A, Sasaguri Y, Kitada S, Kusaka Y, Kuwano K, Masutomi K, Mizuki E, Akao T, Ohba M (2004). A Bacillus thuringiensis crystal protein with selective cytocidal action to human cells. J. Biol. Chem. 279(20):21282-21286.
Crossref

 
 

Jung Y-C, Mizuki E, Akao T, Cote J-C (2007). Isolation and characterization of a novel Bacillus thuringiensis strain expressing a novel crystal protein with cytocidal activity against human cancer cells. J. Appl. Microbiol. 103:65-79.
Crossref

 
 

Katayama H, Kusaka Y, Yokota H, Akao T, Kojima M, Nakamura O, Mekada E, Mizuki E (2007). Parasporin-1, a novel cytotoxic protein from Bacillus thuringiensis, induces Ca2+ influx and a sustained elevation of the cytoplasmic Ca2+concentration in toxin-sensitive cells. J. Biol. Chem. 282(10):7742-7752.
Crossref

 
 

Katayama H, Yokota H, Akao T, Nakamura O, Ohba M, Mekada E, Mizuki E (2005). Parasporin-1, a novel cytotoxic protein to human cells from noninsecticidal parasporal inclusions of Bacillus thuringiensis. J. Biochem. 137:17-25.
Crossref

 
 

Khyami-Horani H (2002). Toxicity of Bacillus thuringiensis and B. sphaericus to laboratory populations of Drosophila melanogaster (Diptera: Drosophilidae). J. Basic Microbiol. 42:105-110.
Crossref

 
 

Khyami-Horani H, Hajaij M, Charles JF (2003). Characterization of Bacillus thuringiensis ser. jordanica (serotype H 71), a novel serovariety isolated in Jordan. Curr. Microbiol. 47:26-31.
Crossref

 
 

Kim H-S, Yamashita S, Akao T, Saitoh H, Higuchi K, Park YS, Mizuki E, Ohba M (2000). In vitro cytotoxicity of non-Cyt inclusion proteins of a Bacillus thuringiensis isolate against human cells, including cancer cells. J. Appl. Microbiol. 89(1):16-23.
Crossref

 
 

Kitada S, Abe Y, Shimada H, Kusaka Y, Matsuo Y, Katayama H, Okumura S, Akao T, Mizuki E, Kuge O, Sasaguri Y, Ohba M, Ito A (2006). Cytocidal actions of parasporin-2, an anti-tumor crystal toxin from Bacillus thuringiensis. J. Biol. Chem. 281(36):26350-2660.
Crossref

 
 

Lee D-W, Akao T, Yamashita S, Katayama H, Maeda M, Saitoh H, Mizuki E, Ohba M (2000). Noninsecticidal parasporal proteins of a Bacillus thuringiensis serovar shandongiensis isolate exhibit a preferential cytotoxicity against human leukemic T cells. Biochem. Biophys. Res. Commun. 272:218-223.
Crossref

 
 

Ministry of Health (MOH), Jordan Cancer Registry (2013). Statistic Summary for Cancer Incidence in Jordan, Non-communicable Diseases Directorate. The 18th Annual Report, Pp. 3-11.

 
 

Mizuki E, Ichimatsu T, Hwang S-H, Park YS, Saitoh H, Higuchi K, Ohba M (1999a). Ubiquity of Bacillus thuringiensison phylloplanes of arboreous and herbaceous plants in Japan. J. Appl. Microbiol. 86:979-984.
Crossref

 
 

Mizuki E, Ohba M, Akao T, Yamashita S, Saitoh H, Park YS (1999b). Unique activity associated with non-insecticidal Bacillus thuringiensis parasporal inclusions: in vitro cell-killing action on human cancer cells. J. Appl. Microbiol. 86:477-486.
Crossref

 
 

Mizuki E, Park YS, Saitoh H, Yamashita S, Akao T, Higuchi K, Ohba M (2000). Parasporin, human leukemic cell-recognizing parasporal protein of Bacillus thuringiensis. Clin. Diagn. Lab. Immunol. 7(4):625-634.
Crossref

 
 

Mosmann T (1983). Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods 65(1-2):55-63.
Crossref

 
 

Nagamatsu Y, Okamura S, Saitou H, Akao T, Mizuki E (2010). Three Cry toxins in two types from Bacillus thuringiensis strain M019 preferentially kill human hepatocyte cancer and uterus cervix cancer cells. Biosci. Biotechnol. Biochem. 74:494-498.
Crossref

 
 

Namba A, Yamagiwa M, Amano H, Akao T, Mizuki E, Ohba M, Sakai H (2003). The cytotoxicity of Bacillus thuringiensis subsp. coreanensis A1519 strain against the human leukemic T cell. Biochim. Biophys. Acta 1622:29-35.
Crossref

 
 

Obeidat M (2008). Molecular typing of local Bacillus thuringiensis strains and determination of their parasporal crystal proteins cytotoxicity against cancer cells. PhD Thesis, University of Jordan, pp. 53-65.

 
 

Obeidat M, Hassawi D, Ghabeish I (2004). Characterization of Bacillus thuringiensis strains from Jordan and their toxicity to the Lepidoptera, Ephestia kuehniella Zeller. Afr. J. Biotechnol. 3(11):622-626.

 
 

Obeidat M, Khyami-Horani H, Al-Momani F (2012). Toxicity of Bacillus thuringiensis β-exotoxins and δ-endotoxins to Drosophila melanogaster, Ephestia kuhniella, and human erythrocytes. Afr. J. Biotechnol. 11(46):10504-10512.

 
 

Obeidat M., Al-Momani F, Saadoun I (2000). Diversity of Bacillus thuringiensis in different habitats of nothern Jordan. J. Basic Microbiol. 40(5-6):385-388.
Crossref

 
 

Ohba M (1996). Bacillus thuringiensis populations naturally occurring on mulberry leaves: A possible source of the populations associated with silkworm-rearing insectaries. J. Appl. Bacteriol. 80:56-64.
Crossref

 
 

Ohba M, Yu YM, Aizawa K (1988). Occurrence of non-insecticidal Bacillus thuringiensis flagellar serotype 14 in the soil of Japan. Syst. Appl. Microbiol. 11:85-89.
Crossref

 
 

Okumura S, Akao T, Higuchi K, Saitoh H, Mizuki E, Ohba M, Inouye K (2004). Bacillus thuringiensis serovar shandongiensis strain 89-T-34-22 produces multiple cytotoxic proteins with similar molecular masses against human cancer cell. Lett. Appl. Microbiol. 39:89-92.
Crossref

 
 

Okumura S, Ohba M, Mizuki E, Crickmore N, Côté J-C, Nagamatsu Y, Kitada S, Sakai H, Harata K, Shin T (2010). Parasporin nomenclature. 

View

 
 

Okumura S, Saitoh H, Ishikawa T, Wasano N, Yamashita S, Kusumoto K, Akao T, Mizuki E, Ohba M, Inouye K (2005). Identification of a novel cytotoxic protein, Cry45Aa, from Bacillus thuringiensis A1470 and its selective cytotoxic activity against various mammalian cell lines. J. Agric. Food Chem. 53:6313-6318.
Crossref

 
 

Plummer M, de Martel C, Vignat J, Ferlay J, Bray F, Franceschi S (2016). Global burden of cancers attributable to infections in 2012: A synthetic analysis. Lancet Glob. Health 4(9):e609-e616.
Crossref

 
 

Roh JY, Park HW, Jin BR, Kim HS, Yu YM, Kang SK (1996). Characterization of novel non-toxic Bacillus thuringiensis isolated from Korea. Lett. Appl. Microbiol. 23:249-252.
Crossref

 
 

Saadoun I, Al-Momani F, Obeidat M, Meqdam M, Elbetieha A (2001). Assessment of toxic potential of local Jordanian Bacillus thuringiensis isolates on Drosophila melanogaster and Culex. J. Appl. Microbiol. 90:1-7.
Crossref

 
 

Saitoh H, Higuchi K, Mizuki E, Ohba M (1996). Larvicidal activity of Bacillus thuringiensis natural isolates, indigenous to Japan, against two nematoceran insect pests occurring in urban sewage environments. Microbiol. Res. 151:263-271.
Crossref

 
 

Saitoh H, Okumura S, Ishikawa T, Akao T, Mizuki E, Ohba M (2006). Investigation of anovel gene encoding a parasporal protein, parasporin-4, that preferentially kills human leukemic T cells. Biosci. Biotechnol. Biochem. 70(12):2935-2941.
Crossref

 
 

Saraswathy N, Kumar P (2004). Protein engineering of δ-endotoxins of Bacillus thuringiensis. Electron. J. Biotechnol. 7(2):178-188.

 
 

Travers R, Martin P, Reichelderfer C (1987). Selective process for efficient isolation of soils Bacillus spp. Appl. Environ. Microbiol. 53(6):1263-1266.

 
 

Uemori A, Ohgushi A, Yasutake K, Maeda M, Mizuki E, Ohba M (2008). Parasporin-1Ab, a novel Bacillus thuringiensis cytotoxin preferentially active on human cancer cells in vitro. Anticancer Res. 28:91-95.

 
 

Wong SYR (2010). Bacillus thuringiensis parasporal proteins and their effect on human cancer cells. Int. eJ. Sci. Med. Edu. 4:3-9.

 
 

World Health Organization (WHO) (2017). Cancer Fact Sheet, February (2017). 

View

 
 

Yamashita S, Akao T, Mizuki E, Saitoh H, Higuchi K, Park YS, Kim H-S, Ohba M (2000). Characterization of the anti-cancer-cell parasporal proteins of a Bacillus thuringiensis isolate. Can. J. Microbiol. 46:913-919.
Crossref

 
 

Yamashita S, Katayama H, Saitoh H, Akao T, Park YS, Mizuki E, Ohba M, Ito A (2005). Typical three-domain Cry proteins of Bacillus thuringiensis strain A1462 exhibit cytocidal activity on limited human cancer cells. J. Biochem. 138:663-672.
Crossref

 
 

Yasutake K, Uemori A Binh ND, Mizuki E, Ohba M (2008). Identification of parasporin genes in Vietnamese isolates of Bacillus thuringiensis. Z. Naturforsch. C. 63:139-143.
Crossref