African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12488

Full Length Research Paper

Callus induction in three mosaic disease resistant cassava cultivars in Benin and genetic stability of the induced calli using simple sequence repeat (SSR) and sequence-characterized amplified region (SCAR) markers

Amitchihoué Franck Sessou
  • Amitchihoué Franck Sessou
  • Institute of Basic Sciences, Technology and Innovation, Pan African University, P. O. Box 62000-00200, Nairobi, Kenya.
  • Google Scholar
Jane W. Kahia
  • Jane W. Kahia
  • Coffee Research Institute, P. O. Box 4, Ruiru, Kenya.
  • Google Scholar
Elijah Ateka
  • Elijah Ateka
  • Jomo Kenyatta University of Agriculture and Technology, P. O. Box 62000-00200, Nairobi, Kenya.
  • Google Scholar
Jerome Anani Houngue
  • Jerome Anani Houngue
  • Laboratory of Biochemistry and Molecular Biology, Faculty of Sciences and Technology (FAST), University of Abomey-Calavi (UAC), P.O. Box 526 Cotonou, Benin.
  • Google Scholar
Colombe Dadjo
  • Colombe Dadjo
  • Institute of Basic Sciences, Technology and Innovation, Pan African University, P. O. Box 62000-00200, Nairobi, Kenya.
  • Google Scholar
Peter Njenga
  • Peter Njenga
  • Jomo Kenyatta University of Agriculture and Technology, P. O. Box 62000-00200, Nairobi, Kenya.
  • Google Scholar
Corneille Ahanhanzo
  • Corneille Ahanhanzo
  • Laboratory of Biochemistry and Molecular Biology, Faculty of Sciences and Technology (FAST), University of Abomey-Calavi (UAC), P.O. Box 526 Cotonou, Benin.
  • Google Scholar


  •  Received: 10 September 2019
  •  Accepted: 31 October 2019
  •  Published: 30 November 2019

References

Acedo V, Labana, C (2008). Rapid propagation of released Philippine cassava varieties through tissue culture. Journal of Root Crops. 34:108-114.

 

Agre AP, Rabbi IY, Battachargee R, Augusto A, Dansi M, Melaku G, Sanni A, Akouegninou A, Akpagana K (2015). Agromorphological characterization of elite cassava (Manihot esculenta Crantz) cultivars collected in Benin. International Journal of Current Research in Biosciences and Plant Biology 2(2):1-14.

 
 

Anuradha T, Kumar KK, Balasubramanian P (2015). Cyclic somatic embryogenesis of elite Indian cassava variety H-226. Indian Journal of Biotechnology 14:559-565.

 
 

Atehnkeng J, Adetimirin VO, Ng SYC (2006). Exploring the African cassava (Manihot esculenta Crantz) germplasm for somatic embryogenic competence. African Journal of Biotechnology 5(14):1324-1329

 
 

Beyene G, Chauhan RD, Wagaba H, Moll T, Alicai T, Miano D, Carrington JC, Taylor NJ (2016). Loss of CMD2-mediated resistance to cassava mosaic disease in plants regenerated through somatic embryogenesis. Molecular Plant Pathology 17(7):1095-1110.
Crossref

 
 

Bronsema FBF, Van Oostveen WJF, Van Lammeren AAM (1997). Comparative analysis of callus formation and regeneration on cultured immature maize embryos of the inbred lines A188 and A632. Plant Cell, Tissue and Organ Culture 50(1):57-65.
Crossref

 
 

Cacai GHT, Ahanhanzo C, Dangou JS, Houedjissin SS, Agbangla C (2012). Effets de différentes combinaisons hormonales sur l'organogenèse in vitro de quelques cultivars locaux et variétés améliorées de Manihot esculenta Crantz (manioc-Euphorbiaceae) cultivées au Bénin. International Journal of Biological and Chemical Sciences 6(4):1593-1607.
Crossref

 
 

Cacaï GHT, Adoukonou-Sagbadja H, Kumulugui BS, Ovono PO, Houngue J, Ahanhanzo C (2013). Eradication of cassava (Manihot esculenta) mosaic symptoms through thermotherapy and meristems cultured in vitro. International Journal of Agronomy and Plant Production 4(Special Issue):3697-3701.

 
 

Cao Z, Sui S, Cai X, Yang Q, Deng Z (2016). Somaclonal variation in 'Red Flash'caladium: morphological, cytological and molecular characterization. Plant Cell, Tissue and Organ Culture 126(2):269-279.
Crossref

 
 

Castro AHF, Braga K de Q, Sousa FM de, Coimbra MC, Chagas RCR (2016). Callus induction and bioactive phenolic compounds production from Byrsonima verbascifolia (L.) DC. (Malpighiaceae). Revista Ciência Agronômica 47(1):143-151.
Crossref

 
 

Chauhan RD, Beyene G, Taylor NJ (2018). Multiple morphogenic culture systems cause loss of resistance to cassava mosaic disease. BMC Plant Biology 18:132.
Crossref

 
 

Chen JT, Chang WC (2006). Direct somatic embryogenesis and plant regeneration from leaf explants of Phalaenopsis amabilis. Biologia Plantarum 50(2):169-173.
Crossref

 
 

Dadjo C, Kahia J, Muthuri C, Diby L, Kouame C, Njenga P, Kouassi M (2015). Induction and regeneration of somatic embryos from Vitex doniana (Lamiaceae) leaf explants. International Journal for Biotechnology and Molecular Biology Research 6(4):28-34.
Crossref

 
 

Danso KE (1997). in-vitro propagation of selected cassava (Manihot esculenta crantz) cultivars using multiple shoot induction and somatic embryogenesis. M. Sc. Dissertation, University of Ghana. pp 132.

 
 

Deo PC, Tyagi AP, Taylor M, Harding R, Becker D (2010). Factors affecting somatic embryogenesis and transformation in modern plant breeding. The South Pacific Journal of Natural Science 28(1):27-40.
Crossref

 
 

Diniz LEC, Ruas C de F, Carvalho V de P, Torres FM, Ruas EA, Santos M de O, Sera T, Ruas PM (2005). Genetic diversity among forty coffee varieties assessed by RAPD markers associated with restriction digestion. Brazilian Archives of Biology and Technology 48(4):511-521.
Crossref

 
 

FAOSTAT: Food and Agriculture Organization of the United Nations. (2017). Agricultural statistics for 2017. FAO. United Nations. 

View

 
 

Fathil NAM, Puad NIM, Amid A, Azmi AS, Ibrahim R (2017). Optimization of plant growth regulators for Citrus suhuiensis callus induction. Asia-Pacific Journal of Molecular Biology and Biotechnology 25(1):75-81.

 
 

Fauquet C, Fargette D, Munihor C (1990). African Cassava Mosaic Virus: Etiology, Epidemiology, and Control. Plant Disease 74(6):404-411.
Crossref

 
 

Faye A, Sagna M, Kane PMD, Sane D (2015). Effects of different hormones on organogenesis in vitro of some varieties of cassava (Manihot esculenta Crantz) grown in Senegal. African Journal of Plant Science 9(8):305-312.

 
 

Feitosa T, Bastos JLP, Ponte LFA, Jucá TL, Campos F de A de P (2007). Somatic embryogenesis in cassava genotypes from the northeast of Brazil. Brazilian Archives of Biology and Technology 50(2):201-206.
Crossref

 
 

Fletcher EKA, Amoako TNE, Twumasi P (2011). Effect of 2, 4-D, explants type and cultivar on the callogenesis expression of cassava (Manihot esculenta Crantz) in Ghana.  African Journal of Biotechnology 10(46):9396-9401.
Crossref

 
 

Fondong VN (2017). The search for resistance to cassava mosaic geminiviruses: how much we have accomplished, and what lies ahead. Frontiers in plant science 8:408.
Crossref

 
 

Guma TB, Jane K, Justus O, Kariuki PN (2015). Standardization of in vitro sterilization and callus induction protocol for leaf explants of anchote: Coccinia abyssinica. International Journal of Research and Development in Pharmacy and Life Sciences 4(2):1427-1433.

 
 

Hahn SK, Isoba JCG, Ikotun T (1989). Resistance breeding in root and tuber crops at the International Institute of Tropical Agriculture (IITA), Ibadan, Nigeria. Crop Protection 8(3):147-168.
Crossref

 
 

Hess JR, Carman JG (1998). Embryogenic competence of immature wheat embryos: genotype, donor plant environment, and endogenous hormone levels. Crop Science 38(1):249-253.
Crossref

 
 

Houngue JA, Zandjanakou-Tachin M, Ngalle HB, Pita JS, Cacaï GHT, Ngatat SE, Bell JM, Ahanhanzo C (2018). Evaluation of resistance to cassava mosaic disease in selected African cassava cultivars using combined molecular and greenhouse grafting tools. Physiological and Molecular Plant Pathology 105:47-53.
Crossref

 
 

Isah BI, Mustapha Y, Sani LA (2018). Effect of types and concentrations of auxins on callus induction and primary somatic embryogenesis in low cyanide cassava cultivars (Manihot esculentum Cranz). Bayero Journal of Pure and Applied Sciences 11(1):497-501.
Crossref

 
 

Jibu T, Kumar RR, Mandal AKA (2006). Metabolite profiling and characterization of somaclonal variants in tea (Camellia spp.) for identifying productive and quality accession. Phytochemistry 67(11):1136-1142.
Crossref

 
 

Jin S, Mushke R, Zhu H, Tu L, Lin Z, Zhang Y, Zhang X (2008). Detection of somaclonal variation of cotton (Gossypium hirsutum) using cytogenetics, flow cytometry and molecular markers. Plant Cell Reports 27(8):1303-1316.
Crossref

 
 

Jiménez VM, Bangerth F (2001). Hormonal status of maize initial explants and of the embryogenic and non-embryogenic callus cultures derived from them as related to morphogenesis in vitro. Plant Science 160(2):247-257.
Crossref

 
 

Jogaiah S, Sharathchandra RG, Raj N, Vedamurthy AB, Shetty HS (2014). Development of SCAR Marker Associated with Downy Mildew Disease Resistance in Pearl Millet (Pennisetum glaucum L.). Molecular Biology Report 41(12):7815-24. 
Crossref

 
 

Le BV, Anh BL, Soytong K, Danh ND, Hong LTA (2007). Plant regeneration of cassava (Manihot esculenta crantz ) plants. Journal of Agricultural Technology 3(1):121-127.

 
 

Leva AR, Petruccelli R, Rinaldi LMR (2012). Somaclonal variation in tissue culture: a case study with olive. Recent Advances in Plant in vitro Culture 7:123-150.
Crossref

 
 

Lin CS, Lin CC, Chang WC (2004). Effect of thidiazuron on vegetative tissue-derived somatic embryogenesis and flowering of bamboo Bambusa edulis. Plant Cell, Tissue and Organ Culture 76(1):75-82.
Crossref

 
 

Lubabali HA (2015). Direct Somatic Embryogenesis of Selected Commercial Coffea Arabica L. Varieties in Kenya. Msc Dissertation, University of JKUAT, Kenya, pp. 1-77.

 
 

Ma G, Xu Q (2002). Induction of somatic embryogenesis and adventitious shoots from immature leaves of cassava. Plant Cell, Tissue and Organ Culture 70(3):281-288.
Crossref

 
 

MAEP: Ministère de l'Agriculture de l'Elevage et de la Pêche (2013). Evolution de réalisations des principales cultures par commune (Période: 1998-2010). MAEP, Benin.

 
 

Magaia HE (2015). Assessment and induction of variability through in vitro mutagenesis in Cassava (Manihot esculenta, Crantz). College of Horticulture, Vellanikkara.

 
 

Mahendran G, Bai VN (2016). Direct somatic embryogenesis of Malaxis densiflora (A. Rich.) Kuntze. Journal of Genetic Engineering and Biotechnology 14(1):77-81.
Crossref

 
 

Mathews H, Schopke C, Carcamo R, Chavarriaga P, Fauquet C, Beachy RN (1993). Improvement of somatic embryogenesis and plant recovery in cassava. Plant Cell Reports 12(6):328-333.
Crossref

 
 

Miché L, Balandreau J (2001). Effects of rice seed surface sterilization with hypochlorite on inoculated Burkholderia vietnamiensis. Applied
Crossref

 
 

and Environmental Microbiology 67(7):3046-3052.

 
 

Mithila J, Hall J, Victor JMR, Saxena P (2003). Thidiazuron induces shoot organogenesis at low concentrations and somatic embryogenesis at high concentrations on leaf and petiole explants of African violet (Saintpaulia ionantha Wendl.). Plant Cell Reports 21(5):408-14.
Crossref

 
 

Mongomake K, Doungous O, Khatabi B, Fondong VN (2015). Somatic embryogenesis and plant regeneration of cassava (Manihot esculenta Crantz) landraces from Cameroon. SpringerPlus 4:477.
Crossref

 
 

Mose W, Indrianto A, Purwantoro A, Semiarti E (2017). The influence of thidiazuron on direct somatic embryo formation from various types of explant in Phalaenopsis amabilis (L.) blume orchid. HAYATI Journal of Biosciences 24(4):201-205.
Crossref

 
 

Murashige T, Skoog F (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum 15(3):473-497.
Crossref

 
 

Okogbenin E, Egesi C, Olasanmi B, Mba C, Kahya S, Hurtado P, Marín JA, Gómez H, De Vicente MC, Fregene MA (2008). SSR analysis of F1 intra specific crosses of cassava for identification of new sources of CMD resistance in African germplasm. Centro Internacional de Agricultura Tropical (CIAT), Cali, CO. 

View

 
 

Omingo DO, Omondi CO, Cheserek J, Runo S, Okun D (2017). Diversity analysis of selected coffee genotypes using microsatellites and random amplified polymorphic DNA in Kenya. International Journal of Biotechnology of Food Sciences 5(1):1-9.

 
 

Onwubiku IOI (2007). SMicropropagation of Cassava {Manihot esculantum Crantz) Using Different Concentrations of Benzyaminiopurine (BAP). Journal of Engineering and Applied Sciences 2(7):1229-1231.

 
 

Osorio M, Gámez E, Molina S, Infante D (2012). Evaluation of cassava plants generated by somatic embryogenesis at different stages of development using molecular markers. Electronic Journal of Biotechnology 15(4):1-11.
Crossref

 
 

Pajević S, Vasić D, Sekulić P (2004). Biochemical characteristics and nutrient content of the callus of sunflower inbred lines/características bioquímicas y el contenido de nutrientes en los callos de las líneas consanguíneas (inbred) de girasol/caractéristiques biochimiques et contenu de nut. Helia 27(41):143-150.
Crossref

 
 

Paran I, Michelmore RW (1993) Development of Reliable PCR-Based Markers Linked to Downy Mildew Resistance Genes in Lettuce. Theoretical and Applied Genetics, 85(8):985-993. 
Crossref

 
 

Park YH, Kim TH, Lee HS, Kim KM, Sohn JK (2010). Morphological and Progeny Variations in Somaclonal Mutants of'Ilpum'(Oryza sativa L.). Korean Journal of Breeding Science 42(4):413-418.

 
 

Phua QY, Chin CK, Asri ZRM, Lam DYA, Subramaniam S, Chew BL (2016). The callugenic effects of 2, 4-dichlorophenoxy acetic acid (2, 4-D) on leaf explants of sabah snake grass (Clinacanthus nutans). Pakistan Journal of Botany 4(2)8:561-566.

 
 

Preece JE (1987). Treatment of the stock plant with plant growth regulators to improve propagation success. HortScience (USA). 

View

 
 

Radhakrishnan R, Ranjitha Kumari B (2008). Morphological and agronomic evaluation of tissue culture derived Indian soybean plants. Acta Agriculturae Slovenica 91(2):391-396
Crossref

 
 

Rajeswari S, Krishnamurthi M, Anand SP, Kumar ST (2009). Performance of somaclones developed from intergeneric hybrids of sugarcane. Sugar Tech 11(3):258-261.
Crossref

 
 

Roels S, Escalona M, Cejas I, Noceda C, Rodriguez R, Canal MJ, Sandoval J, Debergh P (2005). Optimization of plantain (Musa AAB) micropropagation by temporary immersion system. Plant Cell, Tissue and Organ Culture 82(1):57-66.
Crossref

 
 

Sasaki K, Shimomura K, Kamada H, Harada H (1994). IAA metabolism in embryogenic and non-embryogenic carrot cells. Plant and Cell Physiology 35(8):1159-1164.
Crossref

 
 

Sharma N, Gauchan DP, Dhakal A, Luitel A, Shakya S, Shakya R (2015). Establishment of Regenerative Callus, Cell Suspension System and Molecular Characterization of Stevia Rebaudiana Bertoni for the Production of Stevioside in In Vitro. International Journal for Research in Applied Science and Engineering Technology 3(8):133-144.

 
 

Srangsam A, Kanchanapoom K (2003). Thidiazuron Induced Plant Regeneration in Callus Culture of Triploid Banana (Musa sp.) Gros Michel, AAA Group. Songklanakarin Journal of Science and Technology 25(6):689-696.

 
 

Sun QB, Li LF, Li Y, Wu GJ, Ge XJ (2008). SSR and AFLP markers reveal low genetic diversity in the biofuel plant Jatropha curcas in China. Crop Science 48(5):1865-1871.
Crossref

 
 

Vidal ÁM, de Carvalho Costa MAP, da Silva Souza A, de Almeida WAB, Souza FVD (2014). In vitro regeneration and morphogenesis of somatic embryos of cassava. Revista Ciencia Agronomica 45(3):558-565.
Crossref

 
 

Zhang W, Liping Y, Shasha WEI, Deng Z, Jianping YI, Renqi WU, Chen Q (2013) RAPD Marker Conversion into a SCAR Marker for Rapid Identification of Johnsongrass [Sorghum halepense (L.) Pers.]. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 41(1):306-312.
Crossref