African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Partial purification and some physicochemical properties of Aspergillus flavus α-amylase isolated from decomposing cassava peels

Muinat Moronke Adeyanju*
  • Muinat Moronke Adeyanju*
  • Department of Biochemistry, Olabisi Onabanjo University, Ago-Iwoye, Ogun State, Nigeria.
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Oyesola Ojewunmi
  • Oyesola Ojewunmi
  • Department of Biochemistry, College of Medicine, University of Lagos, Lagos, Nigeria.
  • Google Scholar
Temitope Akande
  • Temitope Akande
  • Department of Biochemistry, Olabisi Onabanjo University, Ago-Iwoye, Ogun State, Nigeria.
  • Google Scholar
Esther Nkechi Ezima
  • Esther Nkechi Ezima
  • Department of Biochemistry, Olabisi Onabanjo University, Ago-Iwoye, Ogun State, Nigeria.
  • Google Scholar
Abosede Abolanle Akeredolu
  • Abosede Abolanle Akeredolu
  • Department of Microbiology, Biosciences and Biotechnology, School of Basic and Applied Sciences Babcock University, Ilishan, Ogun State, Nigeria.
  • Google Scholar
Bamidele Sanya Fagbohunka
  • Bamidele Sanya Fagbohunka
  • Department of Biochemistry, Olabisi Onabanjo University, Ago-Iwoye, Ogun State, Nigeria.
  • Google Scholar


  •  Received: 18 August 2014
  •  Accepted: 05 November 2014
  •  Published: 24 December 2014

References

Abe J, Bergman F W, Obeta K, Hizukuri S (1988). Production of the raw starch degrading amylase of Aspergillus sp. K-27. Appl. Microbiol. Biotechnol. 27:447-450.
Crossref
 
Abou-Zeid AM (1997). Production, purification and characterization of an extracellular alpha-amylase enzyme isolated from Aspergillus flavus. Microbios 89:55-66.
 

Aiyer P (2005). Amylases and their applications. Afr. J. Biotechnol. 4(13):1525-1529.

Academic Journals

 
Alariya SS, Sethi S, Gupta S, Gupta BL (2013). Amylase activity of a starch degrading bacteria isolated from soil. Arch. Appl. Sci. Res. 5(1):15-24.
 
Ali FS, Abdel-Moneim A A (1989). Physico-chemical properties of Aspergillus flavus var. columnaris alpha-amylase. Zentralbl Mikrobiol. 144(8):615-21.
 
Ammar YB, Matsubara T, Ito K, Iizuka M, Limpaseni T, Pongsawasdi P, Minamiura N (2002). New action pattern of a maltose-forming alpha-amylase from Streptomyces sp. and its possible application in bakery. J. Biochem. Mol. Biol. 35:568-575.
Crossref
 
Archer DB (2000). Filamentous fungi as microbial cell factories for food use. Curr. Opin. Biotechnol. 11(5):478-483.
Crossref
 
Ashwini K, Gaurav K, Karthik L, Bhaskara Rao KV (2011). Optimization, production and partial purification of extracellular α-amylase from Bacillus sp. Marini. Arch. Appl. Sci. Res. 3(1):33-42.
 
Bernfeld P (1955). Amylases alpha and beta. Methods Enzymol. 1:149.
Crossref
 
Bunni L, Mc Hale L, Mc Hale AP (1989). Production, isolation and partial characterization of an amylase system produced by Talaromyces emersonii CBS 814.70. Enzyme Microb. Technol. 11:370-375.
Crossref
 
Burhan A, Nisa U, Gokhan C, Omer C, Ashabil A, Osman G (2003). Enzymatic properties of a novel thermostable, thermophilic, alkaline and chelator resistant amylase from an alkaliphilic Bacillus sp. isolate ANT-6. Process Biochem. 38:1397-1403.
Crossref
 
Calik P, Ozdamar TH (2001). Carbon sources affect metabolic capacities of Bacillus species for the production of industrial enzymes theoretical analyses for serine and neutral proteases and alpha-amylase. Biochem. Eng. J. 8:61-81.
Crossref
 
Fogarty MW (1983). Microbial Amylases. In Microbial Enzymes and Biotechnology, WM Fogarty (Ed.), Applied Science Publishers Ltd., London, UK. pp. 1-92.
 
Gornall AG Bardawill CJ, David MM (1949). Determination of serum protein by means of the Biuret reaction. J. Biol. Chem. 177: 751-766.
 
Gupta A, Gupta VK, Modi DR, Yadava LP (2008). Production and characterization of α-amylase from Aspergillus niger. Biotechnology. 7(3):551-556.
Crossref
 
Hernández MS, Rodríguez MR, Guerra NP, Rosés RP (2006). Amylase production by Aspergillus niger in submerged cultivation on two wastes from food industries. J. Food Process Eng. 73: 93-100.
Crossref
 
Hmidet N, Bayoudh A, Berrin JG, Kanoun S, Juge N, Nasri M (2008). Purification and biochemical characterization of a novel α-amylase from Bacillus licheniformis NH1 Cloning, nucleotide sequence and expression of amyN gene in Escherichia coli. Process Biochem. 43: 499-510.
Crossref
 
Irfan M, Nadeem M, Syed Q (2012). Media optimization for amylase production in solid state fermentation of wheat bran by fungal strains. J. Cell Mol. Biol. 10(1):55-64.
 
Jensen B, Olsen J (1992). Physicochemical properties of a purified alpha amylase from the thermophilic fungus. Thermomyces lanuginosus. Enzyme Microb. Technol. 14:112-116.
Crossref
 
Jin B, van Leeuwen HJ, Patel B, Yu Q (1998). Utilisation of starch processing wastewater for production of microbial biomass protein and fungal α-amylase by Aspergillus oryzae. Bioresour. Technol. 66:201-206.
Crossref
 
Joel EL, Bhimba BV (2012). Production of amylase from mangrove associated fungi Pestalotiopsis microspora strain VB5 and Aspergillus oryzae strain VB6. Indian J. Geomarine Sci. 41(3):279-283.
 
Kim MJ, Bai D, Ahn B (2011). Identification and characterization of useful fungi with α-amylase activity from the Korean traditional nuruk. Mycobiology 39(4):278-282.
Crossref
 
Knox AM, du Preez JC, Kilian SG (2004). Starch fermentation characteristics of Saccharomyces cerevisiae strains transformed with amylase genes from Lipomyces kononenkoae and Saccharomyces fibuligera. Enzyme Microb. Technol. 34:453-460.
Crossref
 
Miller GL (1959). Use of Dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem. 31:426-429.
Crossref
 
Moller K, Sharif MZ, Olsson L (2004). Production of fungal α-amylase by Saccharomyces kluyveri in glucose-limited cultivations. J. Biotechnol. 111: 311-318.
Crossref
 
Odibo FJC, Ulbrich-Hofmann R (2001). Thermostable α-amylase and glucoamylase from Thermomyces lanuginosus F1. Acta Biotechnol. 21(2):141-153.
Crossref
 
Oliveira AN, Oliveira LA, Andrade JS (2010). Partial Characterization of Amylases of two Indigenous Central Amazonian Rhizobia Strains. Braz. Arch. Biol. Technol. 53(1):35-45.
Crossref
 
Pandey A, Nigam P, Soccol C R, Soccol V T, Singh D and Mohan R (2000). Advances in microbial amylases. Biotechnol. Appl. Biochem. 31:135-152.
Crossref
 
Pandey A, Nigamp VT, Socco L, Singh D and Mohan R (2006). Advances in microbial amylases. Biochemistry 31: 35-152.
 
Parmar N, Singh A, Ward OP (2001). Enzyme Treatment to reduce solids and improve settling of sewage sludge. J. Ind. Microbiol. Biotechnol. 26:383-386.
Crossref
 
Prabakaran M, Thennarasu V, Mangala RA, Bharathidasan R, Chandrakala N, Mohan N (2009). Comparative studies on the enzyme activities of wild and mutant fungal strains isolated from sugarcane field. Indian J. Sci. Technol. 2(11):46-49.
 
Ramachandran S, Patel AK, Nampoothiri KM, Francis F, Nagy V, Szakacs G, Pandey A (2004). Coconut oil cake - A potential raw material for the production of α-amylase. Bioresour. Technol. 93:169-174
Crossref
 
Rao UM, Satyanarayana T (2003). Statistical optimization of a high maltose-forming, hyperthermostable and Ca2+-independent alpha amylase production by an extreme thermopile Geobacillus thermoleovorans using response surface methodology. J. Appl. Microbiol. 95:712-718.
Crossref
 

Reddy NS, Nimmagadda AM, Rao KRS (2003). An overview of the microbial α-amylase family. Afr. J. Biotechnol. 2(12):645-648.

Academic Journals

 
Sajitha N, Vasanthabharathi V, Lakshminarayanan R and Jayalakshmi S (2011). Amylase from an Estuarine Bacillus megaterium. Curr. Res. J. Biol. Sci. 3(2):110-115.
 
Shah IJ, Gami PN, Shukla RM, Acharya DK (2014) Optimization for α-amylase production by Aspergillus oryzae using submerged fermentation technology. Basic Research Journal of Microbiology, 1(4): 01-10.
 
Shuler ML, Kargi F (2002). Bioprocess Engineering: Basic Concepts. 2ndEdn. Prentice Hall.
 
Siddique F, Hussain I, Mahmood M S, Ahmad S I, Rafique A and Iqba A (2014). Descriptions of α-alpha amylase produced from Aspergillus niger. Pak. J. Sci. 66(2):623-631.
 
Sivaramakrishnan S, Gangadharan D, Nampoothiri KM, Soccol RC, Pandey A (2006). α-amylases from microbial sources- an overview on recent development. Food Technol. Biotechnol. 44 (2):173-184.
 
Stryer L (1995). Carbohydrates: Biochemistry (4th Ed.) New York: W H Freeman and Company 18:473.
 
Sudo S, Ishikawa T, Sato K, Oba T (1994). Comparison of acid-stable α-amylase production by Aspergillus kawachii in solid-state and submerged culture. J. Ferment. Bioeng. 77: 483-489.
Crossref
 
Tsukagoshi N, Kobayashi T, Kato M (2001). Regulation of the amylolytic and hemi-cellulolytic genes in aspergilli. JGMA 47:1-19.