February 2015
X-Chromosome short tandem repeat, advantages and typing technology review
Microsatellites of the X-chromosome have been increasingly studied in recent years as a useful tool in forensic analysis. This review describes some details of X-chromosomal short tandem repeat (STR) analysis. Among them are: microsatellites, amplification using polymerase chain reaction (PCR) of STRs, PCR product evaluation, PCR product purification, separation and detection, data analysis of STR by Identity...
February 2015
Bayoud disease of date palm in Algeria: History, epidemiology and integrated disease management
The bayoud is transmitted by the fungus Fusarium oxysporum f. sp. albedinis, which causes drying and rapid die back. To date, the disease has destroyed more than 12 million date palms in Morocco, or two-thirds of the producers of the best dates trees in this country, and three million of palm trees in Algeria with the threat of the spread of this disease to oasis southeast of Algeria. The research on this...
February 2015
Real time PCR mediated determination of the spontaneous occurrence of Sorghum bicolor alleles in wild sorghum populations
The study evaluates the utility of Real Time PCR (RT-PCR) in quantitative and qualitative analysis of alleles in sorghum populations and the spontaneous occurrence of Sorghum bicolor alleles in wild populations of sorghum. Leaf and seed material from wild sorghum accesions were sampled in Homabay, Siaya and Busia counties to represent Western Kenya sorghum producing regions. A second sampling was done on S2...
February 2015
Polyamines and WOX genes in the recalcitrance to plant conversion of somatic embryos of Habanero pepper (Capsicum chinense Jacq.)
In order to determine the role of polyamines in the formation and development of the somatic embryos of Capsicum chinense, the effect of different concentrations (0, 0.01, 0.1, and 1.0 mM) of Putrescine, Spermidine and Spermine on the efficiency and morphology of the embryos was evaluated. The results show that none of the three polyamines evaluated had a significant effect on the number of embryos formed,...
February 2015
Copper-induced changes in growth and antioxidative mechanisms of tea plant (Camellia sinensis (L.) O. Kuntze)
Tea is the oldest, most popular, non-alcoholic caffeine containing beverage in the world. Tea plants are prone to the attack of many diseases which can be controlled by the treatment of pesticides. These pesticides contain heavy metal which prolonged accumulation can lead to the damage of crop yield both in quantity and quality. In the present study, we observed the effects of high concentration of Cu stress...
February 2015
A two-stage decentralised system combining high rate activated sludge (HRAS) with alternating charcoal filters (ACF) for treating small community sewage to reusable standards for agriculture
Water scarcity increasingly drives wastewater recovery. Campaigns towards re-use of wastewater are not very common in Africa among other factors, due to a lack of efficient and cost-effective technology to treat wastewater to re-usable standards. In this study, two treatment systems, a high rate activated sludge (HRAS) system and alternating charcoal filters (ACF) are combined and used to treat wastewater to...
February 2015
Characterization of indole acetic acid endophyte producers in authoctonus Lemna gibba plants from Xochimilco Lake
Xochimilco’s lacustrine zone is a network of channels that, along with the chinampas, conform a unique ecosystem which has served as source of aquatic resources. Duckweeds are small free-floating monocotyledon aquatic plants classified as macrophytes that serve as nutrient pumps and reduce eutrophication effects. Recently, there are number of new studies related to the aquatic plant-microbial interaction focused...
February 2015
Effects of gamma radiation on enzymatic production of lignolytic complex by filamentous fungi
This work aimed to study the effect of gamma radiation on the production of enzymes by filamentous fungi present in the seawater used for thermoelectric Termope S / A, in the vicinity of Port of Suape, Pernambuco. The isolated microorganisms were screened for their ability to produce enzymes. Subsequently, the fungi were irradiated at 3 doses (500, 1000 and 1500 Gy), using 4 inoculation techniques: lyophilisation,...
February 2015
Production of α-amylase by solid state fermentation by Rhizopus oryzae
A thermotolerant strain of Rhizopus oryzae was grown in three agro-industrial by-products: brewers’ rice, corn grits and wheat bran. Different substrates, cultivation time, moisture content, additional nitrogen sources, pH and temperature of incubation were evaluated aiming to optimize growing conditions. The highest enzymatic activity was observed after 24 h of cultivation using wheat bran as substrate with the...
February 2015
Acute oral toxicity and cytotoxicological evaluation of the ethanol extract of Samanea tubulosa pods in Swiss mice
Acute toxicity and cytotoxicity of ethanol extract of Samanea tubulosa (EESt) pods were evaluated in Swiss mice. Acute toxicity studies were conducted based on OECD guidelines 420, where the limit test dose was 5000 mg/kg. Observation was made and recorded systemically for 1, 2, 4 and 24 h after the administration of dose for skin changes, morbidity, aggression and sensitivity of the behavior of the animals. For...
February 2015
Evaluating the antioxidant and anticancer property of Ficus carica fruits
The present study was designed to investigate the antioxidant and anticancer properties of Ficus carica (FC). The dried coarse powder of F. carica fruits was exhaustively extracted with ethanol and the resulting crude ethanolic extract (FCE) was assayed for antioxidant and anticancer activities, since both are inter-related. The antioxidant activity of FCE was determined by reductive potential in reducing ferric ions...
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