December 2011
Establishment of the total RNA extraction system for lily bulbs with abundant polysaccharides
Isolating high quality RNA from lily bulbs is made difficult by the presence of plentiful polysaccharides. To solve this problem, an efficient and steady RNA extraction technique from different parts of scales in three hybrids lily bulbs has been developed through modifications of three common RNA extraction methods, cetyltrimethylammonium bromide (CTAB), sodium dodecyl sulfate (SDS) and Trizol method. NaCl...
December 2011
Gene cloning and characterization of NADH oxidase from Thermococcus kodakarensis
The genome search of Thermococcus kodakarensis revealed three open reading frames, Tk0304, Tk1299 and Tk1392 annotated as nicotinamide adenine dinucleotide (NADH) oxidases. This study deals with cloning, and characterization of Tk0304. The gene, composed of 1320 nucleotides, encodes a protein of 439 amino acids with a molecular weight of 48 kDa. Expression of the gene in Escherichia...
December 2011
Cloning, expression and functional analysis of MAP30 from Momordica charantia reveals its induction of apoptosis of the BGC-823 cells
Momordica Anti-HIV Protein 30 (MAP30) can induce many tumour cells apoptosis, but the mechanism is still unclear. Since the content of MAP30 in Momordica charantia is limited, in this study, the MAP30 gene was cloned and expressed and the induction of the recombinant MAP30 protein on the apoptosis of BGC-823 cells was investigated. Morphological changes were observed by microscopy. The...
December 2011
Over-expression of Sub1A, a submergence tolerance gene from rice, confers enhanced hypoxic stress tolerance in transgenic tobacco plants
Sub1A, an ethylene-response-factor-like (ERE-like) gene, mediates the extinguished submergence tolerance of rice. To gain further insight into the function of Sub1A in other species, we transformed tobacco plants with the gene under the control of the ubiquitin promoter. Compared to the wild-type plants, transgenic plants over-expressing Sub1A exhibited a greater ability to adapt to...
December 2011
Effects of NaCl stress on seed germination, early seedling growth and physiological characteristics of cauliflower (Brassica oleracea L. var. botrytis L.)
Effects of salt stress on seed germination, early seedling growth and some physiological characteristics were evaluated for four cauliflower species in seven treatments of salinity including 0 (control), 34, 68, 102, 136, 170 and 204 mM NaCl in a three replicated randomized completely block design (RCBD). This result showsthat different treatments of salinity had considerable effect on the germination percentage, vigor...
December 2011
Economic evaluation and applications of the policy analysis matrix of sole and intercropping of leguminous and cereals in Shirvan city, Iran
In this study, data collected from North Khorasan Jihad Agriculture and Almanac foreign trade statistics for 2008 to 2009 were used to assess economic and relative advantages of sole and intercropping of millet and bean in Shirvan city by using benefit indicators. By using benefit to costs index, internal rate of return, net present value and policy analysis matrix were calculated. The results show that...
December 2011
Effects of drought and salt stress on seed germination of three leguminous species
The seeds of Medicago sativa (L.), Astragalus adsurgens (Pall.) and Coronilla varia(L.) were evaluated at germination for tolerance to salt (NaCl) and drought conditions induced by polyethylene glycol (PEG) in an experiment of orthogonal design. The results reveal that the germination percentages of M. sativa and A. adsurgens were much higher than that of C....
December 2011
Effects of long-term salinity on the growth of the halophyte Spartina alterniflora Loisel
The effects of salt stress on the growth of Spartina alterniflora were investigated by imposing seven levels of salt stress (0, 100, 200, 300, 400, 500, and 600 mM NaCl) on potted plants. The seedlings were grown in vermiculite in a greenhouse for six months. Optimal growth of S. alterniflora occurred at salinity levels of 100 and 200 mM NaCl. Seedlings grew less in freshwater...
December 2011
A study on reverse osmosis permeating treatment for yarn dyeing effluent using fuzzy linear regression model
This paper presents a fuzzy linear regression model for estimation of reverse osmosis permeating parameters conditions. The proposed model can effectively take on non-crisp, fuzzy and crisp data. This study model used for estimation of reverse osmosis permeating parameters data from Tirupur examines the variables that contribute to the deterioration of membrane. This result demonstrates the capability...
December 2011
Differential nitrate accumulation, nitrate reduction, nitrate reductase activity, protein production and carbohydrate biosynthesis in response to potassium and sodium nitrate
For most of the cultivated crops, nitrate is the major source of nitrogen. Most steps in the nitrate assimilatory pathway are nitrate inducible. In this study, Cucurbita pepowere grown in washed sand per pot at three potassium and sodium nitrate supplies (25, 50 and 100 mM) to investigate the effects of nitrate salts supply on nitrate accumulation, amino acid biosynthesis, total protein production, nitrate...
December 2011
Effects of salt and water stress on plant biomass and photosynthetic characteristics of Tamarisk (Tamarix chinensis Lour.) seedlings
Tamarix chinensis Lour. (Tamarisk) is regarded as a potential shrub that improves the marginal lands, and is conversely considered as an invasive species in many areas. This study conducted controlled experiments to investigate the biomass accumulation, allocation and photosynthetic responses of T. chinensis seedlings to different salt and water conditions. The results showed...
December 2011
Comparative morphological and ecological studies of two Stachys species (sect. Eriostomum, subsect. Germanicae) grown in Turkey
In this study, comparative morphological and ecological studies of Stachys balansaeand S. carduchorum were investigated. Morphologically, general views of taxa and micromorphological features of the trichomes were studied. S. balansae differs fromS. carduchorum in its leaves sericeous-pilose on the upper surface and narrow oblong-lanceolate cauline leaves....
December 2011
The relationship between ancient trees health and soil properties
This study focuses on the representative ancient trees including Pinus bungeanaZucc.ex Endl., Platycladus orientalis (Linn.) Franco, Pinus tabulaeformis Carr.,Sophora japonica Linn. in the Beijing City as these trees have increasingly high cultural and aesthetic values. We employed tree visual assessment to evaluate the level of health of ancient trees. This study also analyzed...
December 2011
Metagenomic analysis of bacterial diversity of Siloam hot water spring, Limpopo, South Africa
The bacterial diversity of Siloam hot water spring was determined using 454 pyrosequencing of two 16S rRNA variable regions V1-3 and V4-7. Analysis of the community DNA revealed that the phyla Proteobacteria, Cyanobacteria, Bacteriodetes, Planctomycetes, Firmicutes, Chloroflexi and Verrucomicrobia were the most abundant. The bacterial diversity detectable and classifiable was greater...
December 2011
Synthesis and biological activities of substituted N'-benzoylhydrazone derivatives
The present study describes the synthesis, antioxidant and antibacterial activities of substituted N'-benzoylhydrazone derivatives, to investigate activities in relation to their SAR. Compounds have been characterised by melting points, MS, 1H NMR and IR spectra. Antioxidant activities were evaluated using hydroxyl radical (OH) and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) scavenging assays and the...
December 2011
Comparative effects of imipramine, sertraline, nifedipine, furosemide and bumetanide on ingestive behaviour in mice
The objective of the study was to evaluate the comparative effects of imipramine, sertraline, nifedipine, furosemide and bumetanide on ingestive behavior in rodents. Twelve groups (with six in each group) of male mice (25 to 35 g) were used in the experiments. They were housed in labelled plastic cages in the departmental laboratory and allowed access to food and water ad libitum. Six groups were treated...
December 2011
Serum insulin-like growth factor-I adult reference values for an automated chemiluminescence immunoassay system
Analysis of insulin-like growth factor I (IGF-I) in serum is fundamental in order to aid in the diagnosis of growth hormone (GH)-related disorders, as well as in the diagnosis and follow-up of other diseases. Considering the importance of IGF-I local normal range, the aim of this study was to determine reference values for IGF-I (Immulite 2000) in a Brazilian adult population from the city of Rio de...
December 2011
Investigation of the protective effects of Vitamin C, iron and desferrioxamine on heat stress resistance in the model organism Caenorhabditis elegans
The aim of this study was to determine the effects of Vitamin C (Vit. C), iron, the iron chelating agent desferrioxamine (DF) and their most effective dose combinations on thermotolerance of Caenorhabditis elegans (C. elegans). Due to the fact that studies on aging and lifespan take long time, thermotolerance was used as a first step analysis for using the time more effectively. The data...
December 2011
Determination of strontium in rat plasma and plasma ultrafiltrate by Zeeman Furnace atomic absorption spectroscopy and its application to a pharmacokinetic study
A rapid and sensitive analytical assay for the determination of total strontium concentrations in rat plasma was developed and validated. The total strontium levels were determined by use of graphite-furnace atomic-absorption spectrometry (GF-AAS) with Zeeman correction, 1000°Ci n ashing temperatures and 2700°C in atomization temperatures after 3.5% HNO3 protein precipitation. The...
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