African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5232

Full Length Research Paper

Effectiveness of temocillin on extended-spectrum beta-lactamase producing Escherichia coli isolates from patients attending the Niamey General Reference Hospital

Alassane Halawen Mohamed
  • Alassane Halawen Mohamed
  • Microbiology Laboratory of the General Reference Hospital (GRH), BP 12674 Niamey, Niger.
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René Dembélé
  • René Dembélé
  • Laboratory of Molecular Biology, Epidemiology and Surveillance of Foodborne Bacteria and viruses (LaBESTA), University Joseph KI-ZERBO of Ouagadougou, 03 BP 7021 Ouagadougou 03, Burkina Faso.
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Laouali Boubou
  • Laouali Boubou
  • Bacteriology Laboratory of the Niamey National Hospital (NNH), BP 238 Niamey, Niger.
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Alio Mahamadou Fody
  • Alio Mahamadou Fody
  • Superior Normal School, University Abdou Moumouni of Niamey, BP: 10662, Niamey, Niger.
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Alix Bénédicte Kagambèga
  • Alix Bénédicte Kagambèga
  • Laboratory of Molecular Biology, Epidemiology and Surveillance of Foodborne Bacteria and viruses (LaBESTA), University Joseph KI-ZERBO of Ouagadougou, 03 BP 7021 Ouagadougou 03, Burkina Faso.
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Hiliassa Coulibaly
  • Hiliassa Coulibaly
  • Laboratory of Molecular Biology, Epidemiology and Surveillance of Foodborne Bacteria and viruses (LaBESTA), University Joseph KI-ZERBO of Ouagadougou, 03 BP 7021 Ouagadougou 03, Burkina Faso.
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Frédéric François Bado
  • Frédéric François Bado
  • Laboratory of Molecular Biology, Epidemiology and Surveillance of Foodborne Bacteria and viruses (LaBESTA), University Joseph KI-ZERBO of Ouagadougou, 03 BP 7021 Ouagadougou 03, Burkina Faso.
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Alkassoum Ibrahim
  • Alkassoum Ibrahim
  • Direction of surveillance and response to epidemics, Ministry of Public Health, Niamey, Niger.
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Eric Adehossi Omar
  • Eric Adehossi Omar
  • Faculty of Health Sciences, University Abdou Moumouni of Niamey BP 12022, Niamey, Niger.
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Nicolas Barro
  • Nicolas Barro
  • Laboratory of Molecular Biology, Epidemiology and Surveillance of Foodborne Bacteria and viruses (LaBESTA), University Joseph KI-ZERBO of Ouagadougou, 03 BP 7021 Ouagadougou 03, Burkina Faso.
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  •  Received: 01 September 2023
  •  Accepted: 05 October 2023
  •  Published: 30 November 2023

Abstract

Extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli urinary tract infections represent a major global health problem, constituting a public health emergency with high patient morbidity and mortality. Temocillin serves as an alternative antibiotic for treating these infections and conserving carbapenems. A total of 209 isolates of uropathogenic Escherichia coli were collected in the Microbiology Laboratory of Niamey General Reference Hospital. The isolates underwent analysis using standard bacteriological methods. Identification and antibiotic susceptibility testing of Escherichia coli isolates were determined using the VITEK-2 system with the GN and AST-N372 cards. Of these isolates, 104 were subjected to ESBL testing. ESBL testing was performed with antibiotic discs of amoxicillin/clavulanic acid (20/10 µg), ceftazidime (30 µg), aztreonam (30 µg), and cefotaxime (30 µg) on Mueller Hinton agar, following the CA-SFM recommendations for 2019. Isolates that did not exhibit an ESBL phenotype were further tested on Mueller Hinton agar with cloxacillin for ESBL associated with AmpC cephalosporinase. Data were recorded and analyzed using EPI INFO software version 7.2.2.6. Microsoft Word and Excel software were utilized for word processing and figure creation. Out of the 209 Escherichia coli isolates, only 104 exhibited ESBL phenotypes, distributed as follows: 91/104 (43.54%) on Mueller Hinton agar and 13/104 (6.22%) on Mueller Hinton agar with cloxacillin, while the remaining 105 isolates did not produce ESBL (50.24%). The sensitivity of ESBL Escherichia coli isolates to temocillin was 62.5%. Temocillin demonstrated good activity against ESBL Escherichia coli isolates. However, it is recommended that empirical treatment with temocillin be included in the guidelines, making it an alternative antibiotic for conserving carbapenems in the treatment of urinary tract infections. Additionally, it can also be used against isolates producing AmpC enzymes.

 

Key words: Extended Spectrum Beta-lactam (ESBL), Escherichia coli, Temocillin, effectiveness, Niger.