African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Optimization of nutritional and physiological conditions for toxigenic strains of Aspergillus flavus

Melvin Samuel. S, and Alka Mehta*
School of Biosciences and Technology, VIT University, Vellore-632014, Tamil Nadu, India.  
Email: [email protected]

  •  Accepted: 08 January 2013
  •  Published: 31 January 2013


The objective of the present work was to evaluate the capacity of two isolates ofAspergillus flavus from sugar cane (SC), SC-3 and SC-5 to produce aflatoxin under different culture conditions. Aflatoxin B(AFB1) production was determined in four different media and culture conditions (temperature and pH) were varied to achieve maximal toxin production. AFB1 was extracted directly from the cultures with chloroform. Thin layer chromatography (TLC) and high performance liquid chromatography (HPLC) were used to identify AFB1. It was observed that A. flavus cultures SC-3 and SC-5 produced highest AFB1 when grown in potato dextrose medium (PD) and maximum mycelium production in sabauroud dextrose medium (SD). Supplementation of potato dextrose medium with peptone (PD+P) and sodium nitrate (PD+SN), AFB1 production increased by 27 and 20% respectively when compared to the control. The effect of pH and temperature on aflatoxin production by A. flavus was examined in peptone containing PD medium. The results concluded that AFBproduction and mycelial growth was maximum at pH 5.6 when incubated at 27°C in potato dextrose medium supplemented with peptone.


Key words: Aflatoxin B1Aspergillus flavus, mycotoxins