African Journal of
Plant Science

  • Abbreviation: Afr. J. Plant Sci.
  • Language: English
  • ISSN: 1996-0824
  • DOI: 10.5897/AJPS
  • Start Year: 2007
  • Published Articles: 809

Full Length Research Paper

Protocol optimization for in vitro propagation of Kulfo, orange flesh sweet potato (Ipomoea batatas) variety using shoot tip culture

Belachew Beyene
  • Belachew Beyene
  • College of Agriculture and Veterinary Medicine, Jimma University, Ethiopia.
  • Google Scholar
Temesgen Menamo
  • Temesgen Menamo
  • College of Agriculture and Veterinary Medicine, Jimma University, Ethiopia.
  • Google Scholar
Gizachew Haile
  • Gizachew Haile
  • Department of Biotechnology, College of Biological and chemical Engineering, Addis Ababa Science and Technology University, Ethiopia.
  • Google Scholar


  •  Received: 02 November 2017
  •  Accepted: 13 February 2018
  •  Published: 31 October 2020

References

Addis N (2013). Low cost micropropagation techniques for sweet potato (Ipomoea batatas (L.) Lam.). M.Sc. Thesis. Addis Ababa University.

 

Benmahioul B, Dorion N, Kaid-Harche M, Daguin F (2012). Micropropagation and ex vitro rooting of pistachio (Pistacia vera L.). Plant Cell, Tissue and Organ Culture108:353-358.
Crossref

 
 

Berihu M (2014). In Vitro Propagation of Sweet Potato (Ipomoea batatas (L.) Lam.) through lateral bud culture. M.Sc. Thesis. Haromaya University.

 
 

Dagne Y, Tewodros M, Asfaw K (2014). Development of High Yielding Taro (Colocacia esculenta L.) Variety for Mid Altitude Growing Areas of Southern Ethiopia. Journal of Plant Science 2:50-54.

 
 

Debergh P, Maene L (1981). A scheme for commercial propagation of ornamental plants by tissue culture. Scientia Horticulturae 14:335-345.
Crossref

 
 

Geleta D, Tileye F (2011). In vitro production of virus-free sweet potato (Ipomoea batatas (L.) Lam) by meristem culture and thermotherapy. Sinet: Ethiopian Journal of Science 34:17-28.

 
 

George E, Hall M, Deklerk G (2008). Plant tissue culture procedure-back ground. In Plant propagation by tissue culture. Springer, 3rd edition, pp. 175-205.

 
 

Gosukonda R, Porobodessai A, Blay E, Prakash C, Peterson C (1995). Thidiazuron-induced adventitious shoot regeneration of sweet potato (Ipomoea batatas). In Vitro Cellular & Developmental Biology-Plant 31:65-71.
Crossref

 
 

Jin X, Wang Y, Zhang Y, Chai T (2008). Rapid in vitro multiplication and ex vitro rooting of Malus zumi (Matsumura) Rehd. Acta Physiologiae Plantarum 30:129-132.
Crossref

 
 

Kataoka I (1994). Influencing of rooting substrates on the morphology of papaya root formed in vitro. Journal of Tropical Agriculture 38:251-257.

 
 

Khadiga G, Rasheid S, Mutasim M (2009). Effect of Cultivar and Growth Regulator on In vitro Micropropagation of Potato (Solanum tuberosum L). American-Eurasian Journal of Sustainable Agriculture 3(3): 487-492.

 
 

Kumar A, Mahindra P, Manoj K, Amit K, Smita S, Shekhawata N (2014). In vitro propagation and ex vitro rooting of Caralluma edulis (Edgew.) Benth and Hook. An endemic and endangered edible plant species of the Thar Desert. Scientia Horticulturae 165:175-180.
Crossref

 
 

Laban TF, Peace K, Robert M, Maggiore K, Hellen M, Muhumuza J (2015). Participatory agronomic performance and sensory evaluation of selected orange-fleshed sweet potato varieties in south western Uganda. Global Journal of Science Frontier Research 15:25-30.

 
 

Mahipal S, Shekhawat N, Kannan M, Manokari C, Ranaweera K, Ranaweera M (2015). In vitro regeneration of shoots and ex vitro rooting of an important medicinal plant Passiflora foetida L. through nodal segment cultures. Journal of Genetic Engineering and Biotechnology 13:209-2014.
Crossref

 
 

Martin K (2003). Rapid in vitro multiplication and ex vitro rooting of Rotula aquatic Loura hoeophytic woody medicinal plant. Plant Cell Reports 21:415-420.
Crossref

 
 

Neja J (2009). Effectiveness of meristem culture and chemotherapy on the production of virus free sweet potato (Ipomoea batatas (L.) Lam.). M.Sc. Thesis. Addis Ababa University.

 
 

Ponnusamy B, Vanstaden J (2013) Rapid in vitro micropropagation of Agapanthus praecox. Research Centre for Plant Growth and Development, School of Life Sciences, University of KwaZulu-Natal Pietermaritzburg, Scottsville, South Africa.

 
 

Ranaweera K, Gunasekara M, Eastward J (2013). Ex vitro rooting: Low cost micro propagation technique for tea (Camellia sinensis (L) O. Kuntz) hybrids. Scientia Horticulturae 155:8-14.
Crossref

 
 

SAS Inistitute (2008). SAS/STAT 9.1 User's Guide the Reg Procedure:(Book Excerpt), SAS Institute.

 
 

Shonga E, Gemu M, Tadesse T, Urage E (2013). Review of entomological research on Sweet potato in Ethiopia. Discourse Journal of Agriculture and Food Sciences 1:83-92.

 
 

Tassew G (2012). In vitro regeneration of sweet potato (Ipomoea batatas (L.) Lam.) from leaf and petiole. M.Sc. Thesis. Addis Ababa University.

 
 

Tekalign W, Tileye F, Girma B, (2012). Meristem culture of selected sweet potato (Ipomoea batatas L. Lam.) cultivars to produce virus-free planting material. Journal of Horticultural Science and Biotechnology 87:255-260.
Crossref

 
 

Tileye F, Welander M, Negash L (2007). Genetic stability, ex vitro rooting and gene expression studies in Hagenia abyssinica. Biologia plantarum 51:15-21.
Crossref

 
 

Tumwegamire S, Kapinga R, Rubaihayo P, LaBonte D, Grüneberg W, Burgos G, Zum Felde T, Carpio R, Pawelzik E, Mwanga R (2011). Evaluation of dry matter, protein, starch, sucrose, β-carotene, iron, zinc, calcium, and magnesium in East African sweetpotato [Ipomoea batatas (L.) Lam] germplasm. HortScience 46(3):348-357.
Crossref