African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12486

Full Length Research Paper

Improvement of the key flavour compounds of fermented garlic by fermentation process optimization

Yunwei Leng
  • Yunwei Leng
  • Department of Biological Engineering, China University of Mining and Technology, Xuzhou, Jiangsu 221008, China.
  • Google Scholar
Shuangshuang Huang
  • Shuangshuang Huang
  • Department of Biological Engineering, China University of Mining and Technology, Xuzhou, Jiangsu 221008, China.
  • Google Scholar
Bing Chen
  • Bing Chen
  • Jiangsu Hansoh Pharmaceutical Co., Ltd., Lianyungang, Jiangsu 222000, China.
  • Google Scholar


  •  Received: 07 September 2020
  •  Accepted: 27 October 2020
  •  Published: 31 October 2020

Abstract

Flavour compounds in fermented garlic were identified by physicochemical analysis and gas chromatography (GC) after pre-treatment. Reducing sugars, acids, polyphenols and 5-hydroxymethyl furfural (5-HMF) were detected in fermented garlic, and 5-HMF was identified to be the key aroma compounds. To further improve the flavour quality of fermented garlic, the influence of pre-treatment method, fermentation temperature and fermentation humidity on the 5-HMF formation was studied, and then single factor tests and response surface methods were employed to optimize the fermentation process. The results showed that the best flavour was obtained when the pre-treatment was done by low temperature freezing, the fermentation temperature was 61.8°C and the humidity was 40.2%. Under the optimum condition, the content of 5-HMF reached 3.6 mg/g with a high quality score of 19.

 

Key words: Flavour enhancement, 5-hydroxymethyl furfural (5-HMF), response surface methodology (RSM).