Study of the antibody stimulation potentials of lactobacillus spp isolated from palm wine

Eze C. O.; Gugu T. H.; Evurani S. A.; Agbo C. P.; Okorie J. E.; Oraeluno J. N.

doi:10.5897/AJB2019.17006

African Journal of
Biotechnology

Abbreviation: Afr. J. Biotechnol.
Language: English
ISSN: 1684-5315
DOI: 10.5897/AJB
Start Year: 2002
Published Articles: 12486

Full Length Research Paper

Study of the antibody stimulation potentials of lactobacillus spp isolated from palm wine

Eze C. O.

Eze C. O.
Department of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmaceutical Sciences, University of Nigeria, Nsukka, Enugu State, Nigeria.
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Gugu T. H.

Gugu T. H.
Department of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmaceutical Sciences, University of Nigeria, Nsukka, Enugu State, Nigeria.
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Evurani S. A.

Evurani S. A.
Department of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmaceutical Sciences, University of Nigeria, Nsukka, Enugu State, Nigeria.
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Agbo C. P.

Agbo C. P.
Department of Pharmaceutics, Faculty of Pharmaceutical Sciences, University of Nigeria, Nsukka, Enugu State, Nigeria.
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Okorie J. E.

Okorie J. E.
Department of Pharmaceutical Technology and Industrial Pharmacy, Faculty of Pharmaceutical Sciences, University of Nigeria, Nsukka, Enugu State, Nigeria.
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Oraeluno J. N.

Oraeluno J. N.
Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmacy, Niger Delta University, Wilberforce Island Amassoma, Bayelsa State, Nigeria.
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Article Number - CD9376B64701
Vol.19(8), pp. 576-583 , August 2020
https://doi.org/10.5897/AJB2019.17006

Received: 14 November 2019
Accepted: 28 July 2020
Published: 31 August 2020

Abstract

In this study lactic acid bacteria were investigated for their presence in fresh palm wine and their effects on humoral immunity examined. The bacteria were isolated from fresh palm wine in a tenfold serial dilution. The isolates were purified by repeated subculture on De Man Rogosa Sharpe (MRS) agar and characterized phenotypically and genotypically. The identified Lactobacillus spp. were investigated for their effects on antibody (humoral immunity) - IgG, IgG1 and IgG2a secretion using sheep red blood cell as antigen (SRBC). Lactobacillus spp. were identified as Lactobacillus brevis, Lactobacillus paracasei subsp. tolerans, Lactobacillus paracasei and Lactobacillus yonginensis. The isolates produced no significant effect on IgG antibody after 4 days’ post-secondary challenge. However, they had significant percentage stimulation at 9 days post-secondary challenge of 100.2, 122.9, 106.4 and 118.3% for L. brevis, L. paracasei sub. tolerans, L. paracasei, and L. yonginensis, respectively. The isolates had marginal and somewhat suppressive effects on IgG1 and IgG2a at both 4 and 9 days’ post-secondary challenge. The results show that fresh palm wine contains Lactobacillus spp. capable of stimulating antibodies production.