November 2011
Inhibitory effect of Cytisus nigricans L. and Cytisus capitatus Scop. on growth of bacteria
In vitro antibacterial activity of ethanol, ethyl acetate and acetone extract from Cytisus nigricans and Cytisus capitatus and their synergistic interaction with gentamicin and cephalexin were studied. Antibacterial efficacy of the extracts was defined by determining minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC). The values were in the range...
November 2011
Optimize culture conditions for biomass and sporulation of the nematophagous fungus Pochonia chlamydosporia, isolate HSY-12-14 by “two-step†cultivation.
Biomass yields and sporulation of Pochonia chlamydosporia isolate HSY-12-14 was concerned on culture conditions including culture method, nutritional requirements together with environmental factors in this study. We optimized them for biomass yields ofP. chlamydosporia HSY-12-14 with the “two-step” cultivation method as well as orthogonal matrix method: firstly...
November 2011
Assessment of some wild Aspergillus species for cellulase production and characterization
Fifteen Aspergillus species were used to evaluate their potentialities for cellulase production under submerged fermentation. The isolates were grown on carboxymethylcellulose (CMC) agar medium for detection of cellulase activity andAspergillus oryzae S/92Gbr exhibited maximum clear zone (40 mm) around the colony. Protein content and cellulase activity of the filtrates were determined and...
November 2011
Bacterial identification and antibiotic susceptibility patterns of Staphyloccocus aureus isolates from patients undergoing tonsillectomy in Malaysian University Hospital
Diagnosis and treatment of chronic tonsillitis (CT) and recurrent tonsillitis (RT) are common problems seen in any otolaryngology clinic. Antibiotic therapy usually fails to prevent the recurrences of these infections leaving the patient with no choice but surgery (tonsillectomy). This study aims to identify the bacteriology of both tonsillar swabs and biopsies specimens among children and adult patients...
November 2011
Antibacterial and antifungal activity of latex of euphorbia antiquorum
Euphorbia antiquorum is a large shrub or small tree belonging to the largest and the most diverse family in the plant kingdom, Euphorbiaceae. In the present study, an attempt was made to evaluate the antimicrobial properties of latex of E. antiquorum on certain microbes. The methanolic extract of the latex was prepared. The extracts were tested for their antibacterial activity against...
November 2011
Biological screening of microbes isolated from soil of ex-tin mining land in Kampar Area
Soil samples from ex-tin mining area located in proximity to Universiti Tunku Abdul Rahman (UTAR), Kampar campus was collected and cultured on nutrient agar plates. The morphological characteristics including basic staining, Gram staining and endospore staining of five selected isolates (SL1-5) were studied. All isolates had circular shape, opaque appearance and smooth surface. SL1, SL2 and SL3 were...
November 2011
Antibacterial activity of Satureja bakhtiarica bung essential oil against some human pathogenic bacteria
Bacterial infections are a major problem in humans; as such, replacing the chemical drugs used against them with plant drugs is an important research. Here, the chemical compositions and antibacterial activity of the essential oils obtained from Satureja bakhtiarica bung against ten human pathogenic bacteria were evaluated. Chemical compositions of essential oil were analyzed by gas...
November 2011
Anti-bacterial activity of in vitro produced alkaloids in Catharanthus roseus L.
Antibacterial therapeutic failure due to emergence of resistant bacterial strain is a world wide phenomenon. The search for effective antibacterial substances from sources such as plants has become a necessity to overcome emergent of bacterial resistant in clinical practice. Catharanthus roseus L. belongs to the family Apocynaceae, is an erector procumbent herb or under shrub containing latex....
November 2011
Nitrogen source affects glutamine synthetase activity in Pantoea sp.; bacterium inoculation promotes rice seedlings’ growth
This study investigated the influence of different sources of nitrogen on Glutamine synthetase (GS) activity of Pantoea sp. strain 9C and the effect of inoculation with the bacterium on the growth medium of rice seedlings. The growth curves of 9C showed lowest cell concentration in LGI medium free of nitrogen and highest cell concentration in LGI medium supplied with 5 mmol/L of serine or...
November 2011
The in vitro antioxidant properties of Bacillus simplex XJ-25 isolated from sand biological soil crusts
XJ-25, a strain with strong antioxidant activity was isolated from sand biological soil crusts in Gurban Tonggut Desert, Xinjiang, China. Strain XJ-25 is closely related to Bacillus simplex through the 16s rDNA sequencing combined with morphological, physiological and biochemical analysis. The medium for optimal antioxidant activity was NB with 1.5 g/Lglucose. Based on the kinetic assay, antioxidant...
November 2011
Community diversity of bacteria and arbuscular mycorrhizal fungi in the rhizosphere of Amorpha fruticosa L., Hippophae rhamnoides L. and Robinia pseudoacacia L. in different ecological regions of Loess Plateau in Shaanxi Province of China
The research of rhizosphere microorganism community structure in degraded areas has been a focus recently. The aim of this study is to analyze the community diversity of bacteria and arbuscular mycorrhizal fungi (AMF) in the rhizosphere of Amorpha fruticosaL., Hippophae rhamnoides L. and Robinia pseudoacacia L. in three different ecological regions of Loess Plateau in Shaanxi...
November 2011
Quantitative changes of serum immunoglobulins A, G and M among patients with Hepatitis A
There is evidence of change in the level of serum immunoglobulins (Ig) in patients with hepatitis A and attempts for these changes to be associated with the clinical course of the disease. The aim of this study is to establish the quantitative changes of IgA, IgG and IgM in hepatitis A patients and to evaluate their significance for the clinical course of the disease. A total of 125 persons...
November 2011
Repellent properties of Nicotiana tabacum and Eucalyptus globoidea against adults of Hyalomma marginatum rufipes
Scientific evaluation of plants for anti-tick properties is a necessary step towards the development of tick control methods that exclude or involve less of synthetic chemicals. In this study, we evaluated the repellent properties of Nicotiana tabacum and Eucalyptusgloboidea against adults of Hyalomma marganitum rufipes using a suitable repellency bioassay. Dichloromethane...
November 2011
Prevalence of equine herpes viruses 1, 2 and 4 in Arabian horse population in Egypt
The Egyptian Arabian horse is one of the oldest and most popular horse breeds in the world. No previous efforts have been made for investigating the existence and prevalence of equine herpes viruses (EHVs) in this precious horse breed. In this report, ninety three clinical samples were collected from a cohort of Arabian horses located in Cairo, Egypt. Screening of the clinical samples for the presence of EHV...
November 2011
Effect of various medicinal plant essential oils obtained from semi-arid climate on rumen fermentation characteristics of a high forage diet using in vitro batch culture
Eighteen natural medicinal plant essential oils and garlic oil obtained from semi-arid climate were conducted to evaluate their effects on in vitro rumen microbial fermentation and their effectiveness for decrease in vitro ruminal methane production. 50 ml of buffered rumen fluid (1:2; rumen fluid: buffer solution) were introduced in 125 ml serum bottles containing 500 mg of 80:20 alfalfa...
November 2011
Effects of lake water chemistry on bacterioplankton community structures of three lakes
To investigate the effects of lake water chemistry on bacterioplankton community composition, three different sized lakes (Lake Taihu, Lake Zixia and Lake Pipa) were studied. Denaturing gradient gel electrophoresis (DGGE) of 16S rRNA, followed by clone library analysis was used to explore the bacterial community structure in lake water. Cluster analysis of DGGE patterns indicated that bacterioplankton...
November 2011
Effect of shRNA-mediated survivin gene silencing on the biological behavior of human bladder transitional cell carcinoma T24 cells
To construct the expression vector of the survivin-specific short hairpin RNA (shRNA) and to investigate its effect on the biological behavior of human bladder transitional cell carcinoma T24 cells. Survivin-specific short oligonucleotides were designed, synthesized, and cloned into the PTZU6+1 vector, resulting in...
November 2011
A comparative study of microbial load, chemical and sensory characteristics of camel meats collected from supermarkets and butcher shops
This study was set out to identify microbial load, chemical, and sensory characteristics of camel meats collected in the winter and summer seasons from both butchery shops and supermarkets butcheries. On day 0, the viable cell count, Pseudomonas count,Streptococcus fecal, coliform fecal count, Staphylococcus count and Staphylococcus aureus were higher in the summer as...
November 2011
Physical adsorption immobilization of antimicrobial peptide (bacitracin) producing bacillus strain GU215 on polystyrene film
The physical immobilization of Bacillus sp GU215 on polystyrene polymer film by adsorption was performed and bacitracin (antimicrobial peptide) production by both free and immobilized cells were investigated at optimum conditions. The immobilized cells presented immediate log time within 4 h, while the free cells showed a 24 h delayed response. Likewise, free cells of Bacillus spp GU215...
November 2011
The systematic characterization of poplar CK2α and its theoretical studies on phosphorylation of P-protein C-terminal domain
Casein kinase 2 (CK2) is a ubiquitous serine-threonine protein kinase, which of specific substrates are involved in the regulation of several vital physiological processes in plants. Detailed studies of CK2 have focused on the herbaceous species of Arabidopsis, maize, however, it has remained largely unexplored in woody trees. In this study, we identified the four genes encoding CK2α subunits in a...
November 2011
Effect of seed and embryo size on early growth of wheat genotypes
Rapid seedling establishment is an important requirement for successful crop production in dry land farming systems. Seed size, as a characteristic of seed quality, influences seedling growth and establishment. In order to study the effects of seed and embryo size on early growth of wheat genotypes the experiment was conducted in a split plot randomized block design with genotypes as main plots and seed size...
November 2011
Cassava solid-state fermentation with a starter culture of Lactobacillus plantarum and Rhyzopus oryzae for cellulase production
A mixture of two microorganisms was used as starter culture, with the aims of producing cellulose from fermented cassava roots. Cellulase yields of 430 IU/g were recorded in solid-state fermentation of cassava starch with mixed culture of Lactobacillus plantarumand Rhyzopus oryzae, isolated from cassava dried chips. High cellulase activity (8.6 IU/ml) per unit volume of enzyme broth and high...
November 2011
Bifidobacterium spp.: A unique etiopathogenic agent for intra-abdominal infections
Bifidobacterium spp. are non-sporulating Gram-positive anaerobic bacilli that can grow also in microaerophilic conditions and are constituting part of normal enteral flora, playing a complex role in the maintenance of the health state of the human organism. However, in recent times, the literature has reported that in certain conditions (advanced age, immunodeficiency status, co-morbidities etc),...
November 2011
Serum lipid profiles and eosinophilia among Giardia cyst passers
Giardia trophozoites attach to upper portion of small intestine and may present with severe damage affecting nutrient absorption. One of the main complications of giardiasis is lipid malabsorption. Consequently, infected people may lack the important caloric source and lipid soluble vitamins. This study aimed to assay serum lipid profiles and eosinophilia in Giardia cyst passers and compare with non- infected...
November 2011
A case of food-borne botulism by consumption of home-made canned red pepper: Importance of the botulism antitoxin therapy
Botulism is a rare but severe neuroparalytic disease, caused by Clostridium botulinumtoxins. Botulinium toxin is one of the most potent toxins in nature. Foodborne botulism is caused by foods particularly canned foods (home/commercial) but also by sausages, meat products and seafoods. Here we described four adult foodborne poisoning cases caused by home-made canned red pepper. Intensive care...
November 2011
The preliminary study of inhibitory mechanism of sulfone compounds xa202 and xt208 against Gibberella zeae
A series of novel methyl sulfone derivatives containing 1,3,4-oxadiazole (thiadiazole) moiety were bioassayed in vitro against Gibberella zeae using mycelial growth rate method. The bioassay results showed that compounds xa202 and xt208 displayed excellent antifungal activity against G. zeae with EC50 values of 3.49 μg/ml and 3.73 μg/ml, respectively. The...
November 2011
Sperm function is affected by the electromagnetic radiation emitted by mobile phone
The use of mobile phone has become more widespread, concerns have mounted regarding the potentially harmful effects of the electromagnetic radiation (EMR) emitted from it. The current study aimed to investigate the effect of the EMR emitted by mobile phone on sperm function. Semen samples were subjected to swim up then separated into two groups. The first group acted as a control group which was unexposed to...
November 2011
Genetic variation and antibiotic susceptibility among Staphylococcus aureus isolates from dairy products and food handlers
A total of one hundred and seventeen isolates of Staphylococcus aureus were obtained from different dairy products and nasal mucosa of cheese factory workers in the state of Jalisco, Mexico. The isolates were characterized by their sensitivity to different antibiotics, while genetic diversity was analyzed by Box-PCR molecular marker. Approximately half (45%) of the isolates analyzed showed...
November 2011
Importance of microbiological fertilizer used in soybean production: Agronomical and biological aspects
Inoculation is the best way to ensure good nodulation with the proper strain of nitrogen-fixing bacteria for increased yield in an environmentally safe manner. This investigation was conducted on three-year experiment which was set up in four replications with three-crop rotation (maize-soybean-wheat) at experimental field of the Institute of Field and Vegetable Crops, Novi Sad, Serbia. Effects of application...
November 2011
Antibacterial and antioxidant activities of the endophytic fungi from medicinal herb Trillium tschonoskii
Fourteen distinct endophytic fungal isolates were first separated from the healthy underground parts of Trillium tschonoskii, which is a traditional medicinal herb mainly distributed in China. They were taxonomically identified by morphological traits and internal transcribed spacer (ITS) rRNA gene sequence analysis. Seven genera,...
November 2011
Extremely low prevalence of intestinal cryptosporidiosis and hygienic practices among hospitalized children with malignancies in Malaysia: A preliminary observation
Intestinal cryptosporidiosis has been reported to be associated with high morbidity and mortality among cancer patients. The objectives of the study were to determine the prevalence of cryptosporidiosis and to elucidate contributing factors that might influence its transmission in our children with cancer. A prospective cross-sectional study among 110 patients hospitalized for chemotherapy and 100 healthy...
November 2011
Statistical optimization of low-cost medium for economical production of Bacillus subtilis biosurfactant, a biocontrol agent for the olive moth Prays oleae
Biosurfactants are currently not a feasible alternative to chemically synthesized surfactants as a result of their potentially high production costs. In this work, Bacillus subtilis SPB1 biosurfactant was shown to be efficient in the biocontrol of the olive mothPrays oleae. Its production was improved by optimizing the medium components using inexpensive substrates. The effect of orange peels, soya...
November 2011
Effect of neem (Azadirachta indica) products on seedling growth of shisham dieback
In present study, the efficacy of different neem (Azadirachta indica A. Juss) products namely neem oil, neem seed decoction, neem seed without coat, neem seed coat and neem leaf extract were tested for in vitro growth of shisham seedlings inoculated withFusarium solani isolated from shisham dieback. Two concentrations (5 and 15%) of each neem product were used in the study. Three different...
November 2011
Quantitative analysis of changes in bacterial aerosols during endodontic, periodontic and prosthodontic treatments
Aerosols are solid or liquid particles which are ejected during dental treatments into air and develop air-borne infection. The aim of this study was to investigate the amount of bacterial aerosols during endodontic, periodontic and prosthodontic treatments. In this study, air samples were collected during three different dental treatments. This sampling was performed at four different distances from the...
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