African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5232

Table of Content: 30 October, 2011; 5(24)

October 2011

Lactobacillus acidophilus cell structure and application

  Lactic acid bacteria (LAB) are one of the most applied bacteria in the production of fermented foods, from dairy to fruits and vegetables products. They make food durable, improve food safety, flavor, and texture as well as to enhance food physiological and hygienic value due to the presence of viable cells and valuable. Besides, products obtained by LAB fermentation processes are of special importance for...

Author(s):   Parvaneh Jafarei and Maryam Tajabadi Ebrahimi

October 2011

Inhibition of Inula cappa (Ham. ex D. Don) DC. extracts on herpes simplex virus infection in vitro

  The methanol and dichloromethane extracts of Inula cappa (Ham. ex D. Don) DC. were evaluated for  inhibition of herpes simplex virus infection. Higher anti- herpes simplex virus (HSV) activity of I. cappa  methanol extract was observed comparing with I. cappa dichloromethane extract since more than 50%  of the virus was inhibited by I. cappa methanol extract and 50% effective doses...

Author(s):   Jiraporn Nikomtat, Puttinan Meepowpan and Yingmanee Tragoolpua 

October 2011

Occurrence of Fusarium species associated with economically important agricultural crops in Iran

  Plant diseases caused by Fusarium spp. will results in yield losses and are becoming more significant  in Iran. In this study, infected plant samples suspected to Fusarium infection, plant debris and  rhizosphere soil were collected from the most important crops that is, wheat, rice, corn, barley, potato,  cucurbits from different provinces in Iran during 2002 to 2009. A total of...

Author(s): K. Chehri

October 2011

Research on the effect of Penicillium sp. on Luzhou-flavor liquor

  Penicillium sp. was isolated from Daqu as a beginner strain. In order to investigate the role of Penicillium sp. in Luzhou-flavor liquor, Penicillium sp. was operated in Koji seeds and then simulated solid-state fermentation for liquor testing under different conditions. They included adding of different amounts of koji seeds and Daqu. The results showed that, when the amount of Daqu added was 20%,...

Author(s): Shangling Fang, Hong Liu , JiangBo Wang and MaoBin Chen

October 2011

Development of a PCR-based method for detection of Nosema pernyi

  Nosema pernyi is the lethal pathogen of pebrine disease in Antheraea pernyi. We have developed a PCR-based method for detection of N. pernyi using specific primers. The primers were designed by the reported conserved regions of microsporidian SSU rRNA. When the genomic DNA of N. pernyi was used as the DNA template, the specific DNA sequences were amplified by PCR. It was observed that PCR diagnosis of N....

Author(s):   Yi-Ren Jiang, Zhen-Hua Deng, Sheng-Lin Shi, Rui-Sheng Yang, Yan-Zhuo Li, Yu-Xi Duan and Li Qin 

October 2011

Theoretical analyses of gene expression for five human pathogens

  Predicted highly expressed (PHX) genes were analyzed in five human pathogens with significant translation selection measured by within-group correspondence analysis (WCA). Functional analysis showed that in the five genomes PHX genes involved in protein synthesis, constitute the largest functional category, followed by categories of energy metabolism and protein fate. The gene encoding pyruvate kinase...

Author(s): Lin Ning and Feng-Biao Guo

October 2011

Effects of some Rhizobium strains on chickpea growth and biological control of Rhizoctonia solani

  Rhizoctonia solani is one of the most important soil-borne fungal pathogens that attack the roots of plant and causes significant damage to different plants particularly to chickpeas (Cicer arietinum L.). The aim of this work is to study the antagonistic activity of different Rhizobium strains against R. solani in dual culture in vitro and under greenhouse conditions. The benefits of rhizobial inoculant in...

Author(s):   Imen HEMISSI,, Yassine MABROUK, Neila ABDI, Manel BOURAOUI, Mouldi SAIDI and Bouaziz SIFI 

October 2011

Rhizobia – As ameliorant to soil reaction

  Soil reaction (pH) is important property soil for crop productions. Many chemicals as ameliorates are required to make soil productive. In organic agriculture, bio-ameliorates are prerequisite. Rhizobia are acid producers and Bradyrhizobia are alkali producers. Rhizobia and Bradyrhizobia are used as ameliorate to study their impact on soil reaction (pH) in laboratory conditions for 21 days. It was observed...

Author(s): Y. P. Dubey

October 2011

Screening of contaminated soils for biodegradable plastic producing bacteria and profiling of their resistance markers

  Polyhydroxyalkanoates (PHA) are the biodegradable polyesters produced by bacteria to overcome the environmental stress. Contaminated environments have a large number of bacteria which can accumulate PHA as their energy reserves. Molasses, oil/ghee and sewerage contaminated soils were selected for the screening of biodegradable plastic- producing bacterial strains. The selected contaminated environments showed...

Author(s): Nighat Naheed, Nazia Jamil and Shahida Hasnain 

October 2011

Production of microbial oils from Mortierella sp for generation of biodiesel livestock

  Biodiesel is an alternative renewable fuel and its production rises rapidly. Microbial oils produced by oleaginous microorganisms can be used as a feedstock for biodiesel production so as to sustain the increasing demand for biodiesel and alleviate the competition with the food supply. This study investigated oil production by an oleaginous fungus, Mortierella sp., which was isolated from soils...

Author(s): Iniya Kumar, M. A. Ramalakshmi, U. Sivakumar, P. Santhanakrishnan and Xinmin Zhan

October 2011

Differentiation of field isolates and vaccine strains of infectious laryngotracheitis virus by DNA sequencing

Avian infectious laryngotracheitis (ILT) is a severe clinical respiratory disease of chickens and causes the clinical symptoms of difficulty in breathing and bloody coughing and as if involves laying hens affect the egg production. Two different regions of the infected cell protein 4 (ICP4) gene of infectious laryngotrucheitis virus (ILTV) were amplified and sequenced for characterization of field isolates and tissue...

Author(s): Majid Sadeghi, Mohammad Hasan Bozorgemehrifard, Hadi keyvanfar, Hassan Momtaz, Abdolhamid Shooshtari and Saied Charkhkar       

October 2011

Vibrio parahaemolyticus’ thermostable direct hemolysin disrupts actin and activates caspase-3 in Rat-1 cells

  In our previous studies, Rat-1 cells treated with Vibrio parahaemolyticus thermostable direct hemolysin (TDH) developed morphological changes including shrinkage and rounding of the cells. Such alterations suggest that TDH stimulates reorganization of the cellular cytoskeleton. In the current study, the effects of TDH on actin cytoskeleton, chromatin, and caspase-3 were evaluated. Rat-1 cells treated with TDH...

Author(s):   Rochman Naim, Fachriyan Hasmi Pasaribu, Tetsuya Iida and Takeshi Honda

October 2011

Exopolysaccharide production by Bacillus cereus GU812900, a fouling marine bacterium

    The aim of the study was to isolate the exopolysaccharides (EPS) from the active biofilm bacterium Bacillus cereus GU 812900 which was isolated from the stainless steel test panel and deployed in the Vellar estuary for a period of about four weeks. The deployed stainless steel test panels when analyzed revealed a direct relationship between the rate of corrosion and the concentration of EPS that...

Author(s): S. Bragadeeswaran, R. Jeevapriya, K. Prabhu, S. Sophia Rani, S. Priyadharsini and T. Balasubramanian

October 2011

Phenotypic and plant growth promoting characteristics of Rhizobium leguminosarum bv. viciae from lentil growing areas of Ethiopia

  Lentil (Lens culnaris Medik.) is an invaluable source of protein for the vast majority of Ethiopian people. It also maintains soil fertility through symbiotic nitrogen-fixation in association with Rhizobium leguminosarum bv. viciae. The aim of this study was selecting symbiotically efficient native lentil nodulating rhizobia endowed with different plant growth-promoting (PGP) characteristics. Hence, 30 lentil...

Author(s): Mulissa Jida, and Fassil Assefa

October 2011

Evaluation of GB virus C (GBV-C)/hepatitis G virus (HGV) and hepatitis type B viruses (HBV) infections in patients with non-Hodgkin’s lymphoma

  GB virus C (GBV-C)/hepatitis G virus (HGV) by infecting and encourage proliferative process of the mononuclear cells, may implicate in lymphomagenesis including non-Hodgkin’s lymphoma (NHL). Therefore, in this study the prevalence of GBV-C/HGV and HBV infections were evaluated in patients with NHL and controls. In a cross sectional study, blood samples were collected from 70 patients with NHL and 100...

Author(s):   Alireza KhodavandiÙ­, Ramin Yaghobi, Fahimeh Alizadeh, Mitra Mirzaee, Mahdi Roshan Nia and Mani Ramzi

October 2011

Studies on the microorganisms associated with dried meat (Tinko) sold in Ilorin, Nigeria

  Dried meat (Tinko) samples obtained from the Oja-Oba market in Ilorin metropolis during the rainy season were investigated for their microbial flora and the sensitivity of the bacterial isolates to different chemotherapeutic antibiotics under two different storage conditions (Cupboard and Refrigerator) for a period of five weeks revealed that the moisture content of dried meat samples stored in the cupboard...

Author(s):   A. E. Ajiboye, O. M. Kolawole, T. O. Oladosu, M. R. Adedayo and J. K. Akintunde

October 2011

Screening for antimicrobial activities of marine molluscs Thais tissoti (Petit, 1852) and Babylonia spirata (Linnaeus, 1758) against human, fish and biofilm pathogenic microorganisms

        The aim of this study was to screen the presence of antimicrobial activities in the marine mollusc Thais tissoti and Babylonia spirata. In this study, the human bacterial pathogen Klebsiella pneumonia (10.02 ± 0.11 mm) and fungal pathogen Aspergillus niger (12.09 ± 0.06 mm) showed most sensitive to ethyl acetate extracts of T. tissoti. In B....

Author(s): N. Sri Kumaran, S. Bragadeeswaran and S. Thangaraj

October 2011

Assessment of the antifungal activities of polyhexamethylene-guanidine hydrochloride (PHMGH)-based disinfectant against fungi isolated from papaya (Carica papaya L.) fruit

  The antifungal activity of the Polyhexamethylene-guanidine hydrochloride (PHMGH)-based disinfectant against fungi isolated from papaya fruit was evaluated. The aim of this work was to show that the PHMGH can be used as a disinfectant for papaya preservation. Thirty one strains of fungi were isolated as Mucor sp., Botrytis sp., Penicillium sp., Geotrichum sp., Aspergillus sp. and Colletotrichum sp. Mucor sp....

Author(s): Rose Koffi-Nevry, Ama Lethicia Manizan, Kablan Tano, Yao Clément Yué Bi, Mathias K. Oulé and Marina Koussémon

October 2011

Galena bio-oxidation by moderate sulphur bacteria

  Bioremediation is a simple and effective technology for metal extraction from low-grade contaminated soils and mineral concentrates. Metal remove from sulfide minerals is based on the activity of mesophilic and chemolithotrophic bacteria, mainly Acidithiobacillus ferrooxidans which convert insoluble metal sulfides into soluble metal sulfates. In this study bioremediation experiments carried out in 1 L...

Author(s): Salari Hassan, Afzali Daryoush and Oliaie Mohammad Sadegh

October 2011

Transportation of single wall carbon nanotube (SWCNT) through the cell membrane

  Carbon nanotubes (CNTs) are very common in medical research and are being highly studied in the fields of biosensing methods for disease treatment and efficient drug delivery and health monitoring. The transportation of open-end Single wall carbon nanotube (SWCNT) through the cell membrane widely investigated because of many advantages. In our recent study, extensive quantum mechanical (QM) calculation of...

Author(s): F. Mollaamin, Sh. Momeni, M. Movahedi and M. Monajjemi

October 2011

Molecular characterization and phylogenetic analysis of neuraminidase gene of avian Influenza H9N2 viruses isolated from commercial broiler chicken in Iran during a period of 1998-2007

  Avian influenza (AI) outbreaks due to H9N2 subtype of avian influenza virus (AIV)  occurred in poultry industry in Iran, throughout the  past  decade and caused serious economic losses as a result of decreased egg production, increased mortality and the cost of vaccination in Iranian poultry industry. Our aim was to find the phylogenic and genetic relationships among avian influenza viruses...

Author(s):   S. Vatandour, M. Bozorgmehrifard, H. Shoushtari, S. Charkhkar and S. Bakhtiari       

October 2011

The effect of GA3 and BA on the quantitative and qualitative characteristics of calla lily (Zantedeschia aethiopica cv. Childsiana)

  Flowering is a complex morphological happening that occurs in apical meristem of stem. This investigation was carried out to study the effects of two hormones including gibberellin (GA) and benzyl adenine (BA) on calla cut flower production. The rhizomes were dipped in GA solution with concentrations include (0, 100, 200 and 500 ppm). BA with concentrations (0, 100, 200 and 500 ppm) was sprayed on flowering...

Author(s):   Najmedin Mortazavi, Rouh-Angiz Naderi, Nasrin Majidian, Behjat Naderi and Yavar Sharafi       

October 2011

Chlorophyll a fluorescence determines the drought resistance capabilities in two varieties of mycorrhized and non-mycorrhized Glycine max Linn

  Arbuscular mycorrhizal fungi, namely, Glomus mosseae and Glomus intraradices, and an endophyte, Piriformospora indica was used to assess the growth of two varieties (JS 335 and TAMS 98-21) of soybean (Glycine max L.) subjected to drought stress. Chlorophyll a fluorescence was measured by Handy PEA (plant efficiency analyzer) and analyzed by Biolyzer programme. The JIP-test (Chlorophyll a fluorescence rise at...

Author(s): Rathod D. P., Brestic M., and Shao H. B.,

October 2011

Experimental vaginal candidiasis: Assessment of Origanum vulgare for its treatment

  The aim of this work was evaluate action of oregano (Origanum vulgare) essential oil in treatment of experimental vaginal candidiasis. A batch of 50 Wistar rats was randomly allocated into four experimental groups corresponding to treatments: T1, 1.5% oil; T2, 3% oil; T3, Nystatin and T4, control treatment (emulsion). Oil concentrations were emulsified in agar suspension at 0.8%. Vulvovaginal candidiasis was...

Author(s):   Marlete Brum Cleff, Iara Wendisch, Angela L. Cabana, Maria Regina Rodrigues, João Roberto Braga de Mello, Mário Carlos Araújo Meireles and Jesús Jaime Hernández-Escareño

October 2011

Evaluation of psychiatric morbidity and quality of life in inactive HbsAg carriers

  Hepatitis B virus (HBV) infection develops in forms from asymptomatic carrier to cirrhosis. There are several studies examining the quality of life of patients with HBV in literature. However, only two studies have investigated the psychiatric morbidity in hepatitis B patients. In this study, we evaluated psychiatric morbidity and quality of life in inactive HbsAg carriers. This case-controlled study was...

Author(s):   Abdulkadir Küçükbayrak, Fatih Canan, Aytekin Alçelik, Gülali AktaÅŸ, Esra KoçoÄŸlu and Tekin TaÅŸ

October 2011

Improvement of bioluminescent assay by combining with the cell growth kinetics for coliforms detection

  The present study focuses on the improvement of limitations of the bioluminescent ATP assay. An attempt was made to improve on the following: non-selective detection, limited cell population density quantification, and to distinguish between bioluminescent noises data and cell ATP, as a suitable technique for coliforms and Escherichia coli detection, as well as other cell strains. Results were achieved by...

Author(s):   Anna Ilyina, Anita J. Casas-Reyes, Karla M. Gregorio-Jauregui, Erika Nava-Reyna, Jose L. Martinez-Hernandez, Nagamani Balagurusamy, Federico Cerda-Ramirez and Yolanda Garza-Garcia       

October 2011

Comparison of the effects of Lactobacillus brevis and Lactobacillus plantarum on cutaneous wound healing in rats

  The benefits of probiotic microorganisms have been tested in several studies and they show many positive effects on human health like reduction of serum cholesterol, stimulation of immune system and prevention or treatment of human infections. This study has shown the activity of Lactobacillus brevis and Lactobacillus plantarum isolated from Iranian traditional cheese on cutaneous wound and describes the...

Author(s):   Zahedi, F., Heydari Nasrabadi, M., Tajabadi Ebrahimi, M. and Aboutalebi, H.  

October 2011

Seroprevalence of Coxiella burnetii in cattle and farm-raised deer in Korea

  Q fever, caused by Coxiella burnetii, is a worldwide zoonosis that affects not wild animals but domestic animals throughout the world, except in New Zealand. Domestic ruminants are considered to be a major infection source of Q fever in humans. However, few studies on the prevalence of Q fever in humans or animals in Korea have been conducted. Thus, the aim of this study was to investigate the seroprevalence...

Author(s):   Yangho Jang, Hyobi Kim, In Heo, Youngjin Park, Sangmo Kim, Myoungheon Lee and  Nonghoon Choe

October 2011

Seroprevalence of Chlamydophila infection in chickens, ducks, geese and pigeons in Southern China

  Chlamydophila psittaci is widely distributed in a number of birds and humans throughout the world, but little is known of the prevalence of C. psittaci in birds in China. In the present study, sera from 240 chickens, 200 ducks, 200 geese and 200 pigeons in China’s southern Guangdong Province were assayed for Chlamydophila antibodies by indirect hemagglutination assay (IHA). The specific antibodies were...

Author(s):   R. Q. Lin, X. Q. Wang, C. Yan, X. H. He, T. Cheng, Y. N. Wang, M. J. Xu, Z. G. Yuan, Y. B. Zhang and X. Q. Zhu,