Molecular characterization of silver resistant E. coli strains isolated from patients suffering from diarrhea
February 2019
Silver nanoparticles (AgNPs) are considered a good alternative for antibiotics due to emerging Multidrug Resistance (MDR) crisis. Resistance to AgNPs is approximately limited among Gram-positive and Gram-negative pathogens. Low toxicity to human cells permits its safe use as a new antimicrobial with broad spectrum. Bacterial cells are used as a factory for AgNPs synthesis supplying a powerful antimicrobial with...
Lactobacillus acidophilus and Bifidobacteria spp having antibacterial and antiviral effects on chronic HCV infection
February 2019
Hepatitis C virus is a serious hepatic disease that could be developed into hepatocellular carcinoma. Previously, some probiotic strains showed a natural therapeutic activity against the fatty liver disorder. Therefore, it could make sense to evaluate the antiviral and antibacterial responses to probiotics as Lactobacillus acidophilus and Bifidobacteria spp. in patients with chronic hepatitis C virus. Twenty (20)...
Antibacterial activities of aqueous and methanol leaf extracts of Solanum incanum Linn. (Solanaceae) against multi-drug resistant bacterial isolates
January 2019
It is estimated that about 80% of the world’s population use medicinal plants either in their crude unmodified form or partially in their modified semi-synthetic form for their medical care. The present study investigated the antibacterial activity of aqueous and methanol leaf extracts of Solanum incanum Linn. (Solanaceae) against multiple drug resistant (MDR) clinical isolates (Streptococcus pyogenes,...
Multiplex RT-PCR identification of five viruses associated with the watermelon crops in the Brazilian Cerrado
January 2019
Watermelon plants can be naturally infected by several viruses in single or mixed infections, of which the diagnosis is difficult and require specific techniques. This study aims to detect and to verify the presence of Cucumber mosaic virus (CMV), Papaya ringspot virus type W (PRSV-W), Watermelon mosaic virus (WMV), Zucchini lethal chlorosis virus (ZLCV), Zucchini yellow mosaic virus (ZYMV), and the main cucurbit...
In vitro activities of aqueous and hydro-ethanolic extracts of Ocimum gratissimum on Escherichia coli ESBL, Klebsiella pneumoniae ESBL and methicillin-resistant Staphylococcus aureus
January 2019
The misuse of antibiotics plays a role in increasing of antimicrobial resistance. The plants, of traditional use, then become an alternative solution to deal with this public health issue. Ocimum gratissimum L. is a widely used plant in Togo for several diseases. The objective of this work was to evaluate the antibacterial activity of the hydro-ethanolic and aqueous extracts of the leaves of this plant, by measuring the...
Evaluation of bacterial culture and their resistant pattern in pus containing patients of different wards of the hospital, Lahore, Pakistan
January 2019
In health care delivery worldwide, infections acquired in health care setting are the most challenging factors. Infections due to antibiotic resistant bacteria have increased day by day in both developed and developing countries. This study is performed to screen the bacterial pathogens present in pus samples taken from patients of different wards of hospital. Isolation of bacteria was determined by standard microbial...
Identification of fliC and flagella expression in Salmonella enterica subspecies enterica serovar Gallinarum biovar gallinarum
January 2019
Salmonella enterica subsp. enterica ser. Gallinarum biovar. gallinarum (S. gallinarum) is the causative agent of fowl Typhoid, a bacterial disease that affects domestic and wild birds. For many years, S. gallinarum was defined as an aflagellated bacterium, that is, non-motile. However, the closely related strain Salmonella pullorum is capable of expressing a flagellum-like filament. Since these bacteria are practically...
Effects of Lactobacillus spp. isolated from the sap of palm tree Elaeis guineensis (palm wine) on cellular and innate immunity
January 2019
The isolation, characterization and effects of lactobacillus spp. from fresh palm wine was undertaken in this study. The aim of this study was to isolate and investigate the in vivo effects of the isolates on innate and cellular immune system. The result of the phenotypic and genotypic characterizations showed the presence of Lactobacillus brevis, Lactobacillus paracasei subsp. Tolerans, Lactobacillus paracasei and...
Study on bovine mastitis with isolation of bacterial and fungal causal agents and assessing antimicrobial resistance patterns of isolated Staphylococcus species in and around Sebeta town, Ethiopia
January 2019
A cross-sectional study was conducted from December 2016 to May 2017 in and around Sebeta town with the aim of assessing the prevalence of mastitis, isolation of aerobic bacterial and fungal causal agents and assessing antimicrobial resistance pattern of isolated Staphylococcus species in dairy cows. From a total of 383 dairy cows, 220 (57.4%) were found to be positive for mastitis of which 10.4% were affected by...
In vitro antibacterial activity of Rumex nervosus and Clematis simensis plants against some bacterial human pathogens
January 2019
Due to quick growth of resistance and high cost of new generation antibiotics, lots of efforts were made to discover new antimicrobial agents from various sources. So, current study was assessed antibacterial activity of ethanol, methanol, acetone, diethyl ether and hexane leave extracts of Rumex nervosus and Clematis simensis by used paper disc diffusion and broth dilution procedures against six human pathogenic...
Evaluation of starter culture fermented sweet potato flour using FTIR spectra and GCMS Chromatogram
January 2019
Starch is the major component of cereal grains and starchy foods, and changes in its biophysical and biochemical properties (such as, amylose, amylopectin, pasting, gelatinization, viscosity) will have a direct effect on its end use properties (such as, bread, malt, polymers). Lactobacillus brevis and Debaromyces polymorphous earlier obtained from fermented sweet poatao broth were used to ferment sweet potato and...
Health and nutritional quality assessment of salmonella-contaminated poultry products in sub-Saharan Africa; A case of cote d'ivoire
December 2018
The poultry sector is developing rapidly in sub-Saharan Africa but it remains informal in many countries, including Côte d'Ivoire. This situation, which is favourable to the health and nutritional insecurity of food of avian origin, deserves special attention, because of Salmonella, which has poultry as its outbreak. The aim of this study was to help reduce the risk of food poisoning linked to the consumption...
Antibacterial activity of endophytic fungus, Penicillium griseofulvum MPR1 isolated from medicinal plant, Mentha pulegium L.
December 2018
In the present study, an endophytic fungus with a considerable antimicrobial activity was isolated from the medicinal plant Mentha Pulegium L. The morphological and molecular identification revealed that this fungus is a Penicillium griseofulvum. A preliminary screening was done to choose the suitable culture medium for a maximum production of the bioactive compounds using the dual-culture agar diffusion assay. The malt...
An evaluation of the effect of graphene oxide on Saccharomyces cerevisiae
December 2018
Graphene oxide (GO) has a wide range of applications. It has been identified to have significant potential for important biomedical and biochemical uses. Graphene and its derivatives are known to elicit toxic effects. Saccharomyces cerevisiae is a versatile yeast of importance with several uses, one of which is its application as a human probiotic. The interaction of GO with living systems is of interest and relevance....
Antimicrobial resistance pattern of MRSA strains isolated from patients of a hospital in Madinah, Kingdom of Saudi Arabia
December 2018
Methicillin-resistant Staphylococcus aureus (MRSA) strains are on the rise leading to severe morbidity and mortality in ICU patients worldwide including Saudi Arabia. In the present study, the prevalence, gender distribution and antimicrobial resistance pattern of this gram-positive nosocomial bacterial pathogen were explored. The strains were isolated from 6840 sampled patients at King Fahd...
Antimicrobial susceptibility patterns of uropathogenic microorganisms associated with vesico-vaginal fistula (VVF) patients in Abakaliki, South eastern Nigeria
December 2018
The objective of this study was to review the antimicrobial susceptibility patterns of uropathogenic microorganisms associated with VVF patients in Abakaliki, South eastern Nigeria. Out of the 117 bacterial isolates obtained from 108 mid-stream urine samples of VVF patients, 72 isolates [15 (20.8%) Staphylococcus aureus, 8 (11.1%) Enterococcus faecalis, 12 (16.7%) Klebsiella pneumoniae, 11 (15.3%) Proteus mirabilis, and...
Isolation and characterization of phenol degrading Bacillus species from a Southeast Brazilian mangrove sediment
December 2018
Phenolic compounds are common pollutants in industrial effluents and pose a significant risk to the environment. The microbial capacity to degrade these molecules makes the prospection of bacteria with this ability very strategic for bioremediation processes in contaminated areas. This study was carried out to isolate bacteria with phenol degrading capacity from Brazilian mangrove soil. Microorganisms were evaluated for...
Seroprevalence of Chikungunya during outbreak in Dhaka, Bangladesh in 2017
December 2018
Chikungunya (CHIK) infection is re-emergence public health problem globally including Bangladesh. It is an arthropod-borne disease, which is transmitted by mosquitoes bite. The virus was first isolated in Newala district of Tanzania in 1953. In 2017, an outbreak of Chikungunya, has struck Bangladesh’s capital, Dhaka. This study was conducted to know the seroprevalence, clinical presentations and seasonal trends of...
Agronomic performance of soybean treated with Bacillus amyloliquefaciens
December 2018
The development of practices that maintain or increases soybean yield can increase the profitability of this crop. In this context, the use of microorganism-based products in crops has been extensively studied. Among the species, Bacillus amyloliquefaciens has shown significant potential for agronomic use due to its ability to control phytoparasitic microorganisms and its effects in promoting plant growth. The aim of...
Diversity and Symbiotic Effectiveness of Rhizobium Isolates Collected from Different Faba bean (Vicia faba) Growing Areas of North and South Gondar, Ethiopia
December 2018
Fifty seven Rhizobium isolates were retrieved based on infection method from different faba bean growing areas of North and South Gondar, Ethiopia. In sand culture, only 35.5% of isolates showed nodulation. Analyses of variance indicated that inoculation of isolates improved tested traits significantly (p< 0.05) at all measured investigated parameters such as shoot length, shoot dry weight, and plant total nitrogen...
Root symbioses in two legume-grass consortia inoculated with soils obtained from degraded coal mining areas in reclamation
November 2018
The intense exploitation of coal deposits in Southern Brazil has caused severe degradation problems in extensive areas. In order to recover such areas, the use of revegetation with species adapted to disturbed environments and with the capacity to establish mutual relationships with microorganisms have been of great value. The objective of this study is to characterize plant-root symbioses in two grass-legume...
Epidemiology, prevalence and antibiotic susceptibility profiles of methicillin-resistant Staphylococcus aureus in farm animals and farm workers in the Central Region of Ghana
November 2018
Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most important microorganisms which has increasingly become resistant to most commonly used antimicrobials. This research investigated the epidemiology, nasal carriage prevalence and the antibiotic susceptibility profile of MRSA among farm animals and farm workers in the Central Region of Ghana. A total of 396 nasal swabs were collected from farm animals...
Diagnosis of Trichomonas vaginalis using real-time polymerase chain reaction (PCR) among women at Institut Pasteur of Côte d’Ivoire
November 2018
Trichomoniasis is a sexually transmitted disease caused by a mobile flagellate pathogenic protist protozoan of the urogenital tract, Trichomonas vaginalis. No data is available in Côte d’Ivoire about its diagnosis by molecular methods. This study aims to identify T. vaginalis by polymerase chain reaction (PCR) among women at Institut Pasteur of Côte d’Ivoire. Vaginal swabs were obtained from each...
Epidemiology of bloodstream infection in Saudi Arabia
November 2018
Bloodstream infections are a challenge to clinicians due to high rates of morbidity and mortality. Moreover, rational antimicrobial therapy, if promptly started, can improve the prognosis of sepsis. The gold-standard blood culture has some limitations and it takes time to complete (about 2-5 days). This leaves the clinicians to start immediately broad-range antimicrobial therapy until microbiology culture’s...
Impact of faecal bacteria contamination on drinking water supply in Aghien Lagoon, Abidjan, Ivory Coast
November 2018
In developing countries, urban surface waters are particularly affected by faecal pollution from domestic wastewaters due to the lack of sanitation and wastewater treatment plants. The presence of pathogenic microorganisms limits the uses of these waters for recreation and economic activities. In Ivory Coast, due to the important gap between water demand and water supply in urban areas, use of surface waters for the...
Diversity of culturable endophytic fungi of Hevea guianensis: A latex producer native tree from the Brazilian Amazon
November 2018
Hevea guianensis is a species of rubber tree native to the Amazon rainforest. This tree is highly exploited for latex extraction but is not cultivated. Therefore, few studies have investigated its microbiota. The aim of this study was to analyze the diversity of endophytic fungi in the leaves, stems and roots of H. guianensis trees from the Brazilian Amazon. A total of 92 fungi were isolated from different tissues of...
Phenotypic and molecular characterization of inducible clindamycin resistance among Staphylococcal strains isolated from cancer patients with febrile neutropenia
November 2018
This work aimed at the phenotypic and molecular characterization of inducible clindamycin resistance among strains of Staphylococcus aureus isolated from cancer patients with febrile neutropenia. Out of 231clinical specimens Staphylococci were isolated from 179 (77.48%) cases. Isolates were identified by conventional microbiological methods. Antimicrobial sensitivity to all isolates was done using disc diffusion...
Antibiotics resistance patterns of Panton-Valentine leukocidin-positive methicillin-resistant staphylococci isolated from clinical samples in Abidjan, Côte d’Ivoire
November 2018
Methicillin-resistant staphylococci have emerged as significant pathogens which cause various infections and its multidrug resistance is a major concern. This study aimed to determine the prevalence of Panton-Valentine leukocidin (PVL) gene and antibiotic resistance patterns of staphylococci isolated from clinical infections in Abidjan. A total of 35 staphylococci strains was obtained from 35 clinical samples (pus,...
In vitro study on the anti-microbial efficacy of Aloe vera against Candida albicans
October 2018
Oral Candida infections are common among the people who are immunocompromised, with poor oral hygiene, diabetics and advanced age. Even though several effective antifungal agents are available for treating oral Candida infections, on prolonged and frequent treatment with these drugs, they may result in development of drug resistant organisms. This leads to looking for an effective, inexpensive and simple alternative....
Antibacterial activity of Ocimum gratissimum (scent leaf) on some pathogenic gastrointestinal bacteria
October 2018
The emergence of antibiotic resistance as well as the recent undesirable side effect of some of the commercially available antibiotics has led to the screening of plant extract in order to discover new drug that could serve as alternative therapy for the treatment of various infections and diseases. Fresh leaf of Ocimum gratissimum (scent leaf) sample was collected, air-dried at room temperature and blended to powder...
Bio-preservative potential of lactic acid bacteria metabolites against fungal pathogens
October 2018
Bio-preservative potential of the secondary metabolites produced by lactic acid bacteria (LAB) isolated from fermented cassava was assessed against fungal pathogens associated with spoilage of fresh fruits and vegetables. Twenty LAB isolates were identified according to standard morphological and biochemical methods and ten were subjected to phenotypic and genotypic identifications. The metabolites produced were tested...
Detection of Escherichia coli and total microbial population in River Siran water of Pakistan using Emb and Tpc agar
October 2018
There is an increased ratio of gastrointestinal diseases in the area of Mansehra, Pakistan, which depends on the Siran River for drinking. These diseases drew our attention to analyze the microbial population. For detection of Escherichia coli and other microbial population, samples were collected from different regions of Mansehra, Pakistan. Two samples were collected from each site for proper detection of...
Antimicrobial, antiparasitic and antiproliferative effects of the extract of Bacillus safensis SG-32 isolated from a Brazilian oil reservoir
October 2018
Strains previously isolated from offshore oil reservoirs in Campos Basin (RJ, Brazil) and Potiguar Basin (RN, Brazil) were tested for production of antimicrobial compounds. Sixteen bacteria produced compounds with antimicrobial activity. The strain chosen for further study was Bacillus safensis SG-32 based on its ability to inhibit 76% of the strains tested. The bacterium was grown in Marine Broth (MB) and the hexane...
Candida albicans interdigital foot infection: A case report highlighting the importance of antifungal susceptibility testing
September 2018
Candida species are opportunistic fungal pathogens which are often isolated from immunocompromised individuals. Candida albicans is the most frequently isolated species in both superficial and invasive candidiasis. Non-albicans species are equally striving in their pathogenic ability, but it is noticed that C. albicans continues to exert its relevance as the leading cause of candidiasis. This is confirmed by this case...
Indigenous arbuscular mycorrhizal fungi associated with tree species of the agroforestry systems of Rwanda and their potential to colonize maize roots
September 2018
Arbuscular Mycorrhizal Fungi (AMF) form symbiotic associations with plant roots and can help mobilize nutrients from soil to the plant. The current study hypothesized that agroforestry systems of Rwanda harbor AMF with the potential to colonize roots of crops and hence enhance productivity. AMF spores were extracted from soil samples collected around most dominant tree species in Bugesera and Rubavu districts,...
Optimization of culture conditions for Paecilomyces lilacinus (Thom) Samson M-14
September 2018
Paecilomyces lilacinus is known as an effective parasite on nematodes which cause diseases to plants. P. lilacinus shows potential as a biocontrol agent against plant parasitic nematodes. The objective of this study is to optimize culture methods including nutritional requirements and environmental factors. The optimized culture conditions for biomass yields of P. lilacinus M-14 were spore suspension on basal medium...
Handling conditions and microbial contamination of fish from Ouagadougou markets in Burkina Faso
September 2018
The purpose of this study was to analyze the handling conditions and microbial contamination of fish from Ouagadougou markets. In spite of the fact that fish consumption is linked to several events of alimentary infection in Burkina Faso, fewer studies were dedicated to this issue. The study was conducted using two approaches. First, an investigation conducted with fish sellers to evaluate handling hygienic conditions...
Microbial contamination of the non-alcoholic beverage Gnamakoudji made from Zingiber officinale in Daloa, Côte d’Ivoire
September 2018
Street food has become unavoidable in the current scheme of restoration in urban Africa. This study aimed to study the microbiological contamination of a refreshing artisanal drink, Gnamakoudji, made from Zingiber officinale sold in the city of Daloa (Côte d’Ivoire). Physicochemical tests showed that Gnamakoudji is an acidic drink (pH ≈ 3.83) and very sweet (0.2 gmL-1). Microbiological analyses...
Isolation and characterization of actinomycetes with antimicrobial activity from the soil and the effect of the environmental factors on their antimicrobial activity
September 2018
This study aimed at isolating and characterizing antibiotic producing actinomycetes from a specific geographic location Alba’qa, Jordan, with a known soil character, and study the effect of the environmental factors on their antimicrobial activity, in order to relate the actinomycetes flora to the environmental characteristics. Soils of the Alba’qa region are of uniform composition and contain antimicrobial...
Predicting maize and soybean crops dry biomass through rhizobacteria microorganisms activity on foliar bio-fertilizer in an arid agro-climate: A multiple linear regression analysis
September 2018
After previously showing rhizobacteria Pseudomonas fluorescence and foliar bio-fertilizer mixture as a suitable practice in improving maize and soybean crops development and dry biomass, this study thus embark on predicting the above-mentioned plants dry biomass feature through their yield and growth parameters by assessing P. fluorescence activities on foliar bio-fertilizer in an arid agro-climate. For this purpose,...
Screening of bacteria isolated from the rhizosphere of maize plant (Zea mays L.) for ammonia production and nitrogen fixation
September 2018
Eleven (11) rhizobacteria identified as Azospirillum sp. isolated from the rhizosphere of maize plants grown in Ibadan, Oyo State, Nigeria were evaluated for ammonia production and nitrogen fixation. The micro-Kjeldahl method was used for the screening of the isolates for nitrogen fixation. Nitrogenase activity ranging from 1.20 to 10.60% was detected in seven of the eleven isolates. Results show that treatments with...
Isolation and characterization of pea plant (Pisum sativum L.) growth-promoting Rhizobacteria
September 2018
Plant growth-promoting rhizobacteria (PGPR) are well-known to influence plant growth via a variety of mechanisms such as nitrogen fixation, production of volatile organic compounds and enzymes, and bioremediation contaminants from the environment. PGPR have been previously identified by other researchers using laboratory screening methods. It was hypothesized that relying on these routine laboratory tests, some PGPR...
Detection of β-tubulin gene from benomyl sensitive isolates of Colletotrichum gloeosporioides causing anthracnose disease in mango
September 2018
Twenty six isolates of Colletotrichum gloeosporioides from anthracnose infected mango fruits were isolated from different places of Tamil Nadu, India and these isolates were identified as C. gloeosporioides by Internal transcribed spacer (ITS) and species specific (CgInt) primers. The sensitivity of C. gloeosporioides isolates to benomyl fungicide were evaluated at five different concentrations viz., 0.5, 1, 2, 5, 10...
Molecular phylogeny and genetic diversity studies of some potent endophytic fungi isolated from medicinal plants (Calotropis procera and Catharanthus roseus) using 18S rRNA and RAPD analysis
September 2018
Molecular identification of four endophytic fungi (Penicillium singorense, Curvularia geniculata, Aspergillus neoflavipes and Alternaria alternata) from two important medicinal plants, Calotropis procera (L.) R.Br. and Catharanthus roseus (L.) G.Don. was carried out by 18SrRNA sequencing. Genetic diversity using two RAPD primer viz. OPB-07 and OPC-06 showed good result with high polymorphism between the four strains....
Sero-prevalence of hepatitis E virus (HEV) genotype 3 in goats from Sokoto Metropolis, Nigeria
September 2018
Hepatitis E virus (HEV) is a cause for public health concern in many developing countries where sanitation conditions are poor. Increasing attention has been focused on the zoonotic nature of HEV. Goats, along with other species of small ruminants have been reported to be susceptible to HEV infection. This study was designed to determine the infection rate of HEV3 by detecting antibodies against capsid proteins of HEV...
Assessment of dominant bacterial strains isolated from Ntoba mbodi, an indigenous African alkaline-fermented food, and their potential enzyme activities
August 2018
Ntoba mbodi is a stable and long-life alkaline-fermented food and the domestic-scale production depends upon microorganisms from the local environment. Previous studies on bacteria in Ntoba mbodi have reported the presence of Bacillus related species and other bacterial taxa. But the abundance of these bacteria in Ntoba mbodi still needs to be determined to assess their ecological importance in this particular...
Molecular detection and characterization of Escherichia coli, Salmonella spp. and Campylobacter spp. isolated from broiler meat in Jamalpur, Tangail, Netrokona and Kishoreganj districts of Bangladesh
August 2018
The study was conducted to isolate, identify and characterize bacterial samples from broiler meat collected from 20 different upazilla markets of Jamalpur, Tangail, Netrokona and Kishoreganj districts of Bangladesh. A total of 20 samples were subjected to bacteriological isolation and identification, and the isolated bacteria were subjected to antimicrobial susceptibility testing using disc diffusion method. Among the...
Screening of human diarrhoeal samples in Mymensingh city of Bangladesh for the isolation, identification and antimicrobial resistance profiles of Campylobacter spp.
August 2018
Campylobacter spp. (Campylobacter jejuni and Campylobacter coli) are one of the major cause of food-borne bacterial diarrhoea in human worldwide. This study was conducted for the isolation, identification and antimicrobial resistance profiling of Campylobacter spp. from diarrhoeal samples of human collected from Surya Kanta (SK) hospital, Mymensingh Medical College, Mymensingh during the period of August 2016 to October...
Isolation and characterization of starch degrading rhizobacteria from soil of Jimma University Main Campus, Ethiopia
August 2018
Starch degrading bacteria are important for different industries such as food, fermentation, textile, and paper. The aim of this study is to isolate and characterize bacteria able to degrade starch from the rhizospheres of various plants at four sites located in Jimma University main campus. Collected soil samples were labeled as kobo (AJUMC), Avocado (BJUMC), Banana (CJUMC), and Cana indica (DJUMC) respectively. Soil...
Low accuracy of the McFarland method for estimation of bacterial populations
August 2018
The McFarland method is designed to estimate bacterial concentrations by means of a turbidity scale (absorbance) which consists of a series of tubes previously calibrated, and with an optical density produced by the precipitation of barium sulphate. This absorbance is compared to bacterial populations. The most used absorbance is the one corresponding to 0.5 on that scale, which assumes a population of 1.5×108...
Page 7 of 105, showing 50 records out of 5215 total, starting on record 301, ending on 350
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