Survey on efficiency of inoculation methods of Pseudomonas fluorescens on growth and yield of Thymus kotschyanus
November 2021
Thyme is one of the most important medicinal plants in wild rangeland in Iran that has lots of benefits. Pseudomonas flourescence is one of the effective plant growth promoting bacteria (PGPR) as a bioinoculant for medicinal plants. This study aims to survey three inoculation techniques with PGPR on growth and oil content in Thymus kotschyanus for organic cultivation. For this research, an experiment...
Seroprevalence and behaviour at risk of zoonotic transmission of bovine brucellosis in Namentenga Province, Burkina Faso
November 2021
Bovine brucellosis is a major zoonosis caused by Brucella abortus. It is a disease with a high hygienic and economic impact that mainly affects breeders, handlers and consumers of dairy products and animal health professionals. It is therefore a real public health problem. The objective of this study was to assess seroprevalence and risk behaviour for zoonotic transmission of bovine brucellosis in Namentenga Province in...
RAPD-based evaluation revealed genetically diverse populations of Pseudomonas aeruginosa and Staphylococcus aureus lytic bacteriophages isolated in urban sewage and Ebrie Lagoon, Côte d’Ivoire
October 2021
The application of bacteriophages biocontrol requires the formulation of genetically distinct bacteriophages in a phage cocktail. Random Amplified Polymorphic DNA (RAPD) - PCR is considered a cheap, reproducible, and readily applicable tool in detecting phage diversity compared to other molecular techniques such as whole-genome sequencing. We used in this study the RAPD-PCR technique to assess the genetic diversity of...
Rabies virus in biting dogs and behaviour at risk of zoonotic transmission of rabies in Ouagadougou, Burkina Faso
October 2021
Rabies is an infectious, viral disease, usually inoculable by dog bite and common to humans and other mammals. It is a major zoonosis but neglected especially in Africa. However, it is the most serious and feared zoonosis in the world because once declared it leads inevitably to death. The objective of this study was to search for rabies virus in biting dogs received in 2020 at the veterinary clinic of the National...
Characterization of bacterial strains from bacterial culture collection of rice sheath in Burundi highlights an Alcaligenes species strain with antibacterial activity against Pseudomonas fuscovaginae rice pathogen
October 2021
The sheath rot rice disease is known to be associated to Pseudomonas fuscovaginae and a recent study on the symptomatic of sheath rot revealed that this bacterial pathogen is abundant in highland rice ecology cultivation in Burundi. From asymptomatic samples of sheath rice, a collection of bacterial isolates and culturable microbiome have been carried out. A comparison between the culturable microbiome and total...
Prevalence of SEA and SEB producing Staphylococcus aureus isolated from foodborne- outbreaks in Iran
October 2021
Staphylococcus aureus is an important pathogen causing a wide variety of diseases such as skin infections, food poisoning, pneumonia and septicemia in humans and animals. Among the bacterial agents of food poisoning, S. aureus is the most common agent worldwide. The aim of this study was to assess prevalence of SEA, SEB enterotoxin producing S. aureus isolated from foodborne outbreaks in Iran. In this study, 313...
Q fever in Tunisia, an underestimated infection
October 2021
Q fever is a common zoonosis with almost a worldwide distribution caused by Coxiella burnetii. Farm animals and pets are the main reservoirs of infection and transmission to humans is usually via inhalation of contaminated aerosols. Infection in humans is often asymptomatic, but it can manifest as an acute disease (usually a self-limited flu-like illness, pneumonia or hepatitis) or as a chronic form (mainly...
Biosafety, bacteriological quality and strategy of biopreservative administration for controlling spoilage bacteria in Thai traditional dried seafood products
October 2021
Biosafety has currently become an important public health issue in seafood industry of Thailand. In order to enhance quality of seafood products, three phases of study were established. In the first phase, biosafety of traditional processed squid sold in Chon Buri province, Thailand compiled from literatures between 2002 and 2019 was evaluated. The squid products represented a health hazard due to 86.5% samples, having...
Prevalence of aflatoxin M1 in pasteurized and ultra-high temperature (UHT) milk marketed in Dar es Salaam, Tanzania
September 2021
The aim of this study was to determine the level of aflatoxin M1 (AFM1) in pasteurized and UHT milk marketed in Dar es Salaam, Tanzania. AFM1 in pasteurized milk samples (75) and ultra-high temperature (UHT) milk (43) was determined by using immuno-affinity high performance liquid chromatography. AFM1 was detected in 97% (115/118) of samples that consisted of 96% (72/75) of pasteurized milk samples and 100%...
Hazard assessment and resistance profile of Escherichia coli strains isolated from bovine carcasses at the main slaughterhouses of Dakar, Senegal
September 2021
Foodborne bacterial hazards and antibiotics resistance are a public health threat. This study aimed at assessing the slaughter conditions, the bacteriological quality of bovine carcasses and at determining the antimicrobial resistance of Escherichia coli strains isolated from bovine carcasses. A questionnaire was administered to 41 stakeholders on the slaughter line. Swabbed carcasses were analyzed according to the...
Human immunodeficiency virus type 1 (HIV-1) subtype diversity in Busia, Western Kenya
September 2021
HIV infection is currently the single biggest epidemic globally. HIV the etiologic agent for AIDS is divided into two types: HIV-1 and HIV-2. HIV-2 is rare and is mainly found in some parts of West Africa. HIV-1 accounts for most cases of AIDS reported globally. HIV-1 strains can be classified into four groups: The "major" group M, group O, group N and the most recent group P. All of which may represent...
Candida spp. associated with hot beverages of coffee and tea sold on street in Côte d’Ivoire
September 2021
Some Candida species are considered as human opportunistic pathogens and can play an important role in spontaneous fermentations, but also as beverage-spoiling microorganisms. The aim of this pioneering study was to investigate Candida spp. in hot beverages, which is consumed mostly on the streets in Côte d’Ivoire. Yeast strains were isolated from 400 hot beverages of tea (200) and coffee (200) samples....
Detection of Legionella pneumophila as the cause of atypical pneumonia in the water sources of the holy places of Makkah
August 2021
Legionella pneumophila is an important pathogen and is involved in more than 95% of cases of severe atypical pneumonia. The current study focused on the ablution water in the grand mosque in Makkah as well as water tanks in hotels surrounding the holy mosque. A total of 100 water samples were collected from ablution water in the Haram and the hotels in the central area around the grand mosque and sent to the...
Aflatoxigenic potential of Aspergillus section Flavi isolated from maize seeds, in Burkina Faso
August 2021
The frequency of occurrence and four principal kinds of aflatoxin concentration in maize seeds grown in Burkina Faso was investigated. Ten (10) samples collected, were analyzed by high performance liquid chromatography (HPLC) with post-column derivatisation after immunoaffinity column cleanup. Eight strains of Aspergillus section Flavi were previously isolated from these samples and cultivated on “Aspergillus...
Biopreservative application of bacteriocins obtained from samples Ictalurus punctatus and fermented Zea mays
August 2021
This study evaluated the preservative ability of protein-like cell free supernatants produced by lactic acid bacteria (LAB) isolates from samples of Ictalurus punctatus (Cat fish) and slurry of fermented Zea mays (Ogi). The LAB strains were separately isolated from understudied samples using De Man, Rogosa and Sharpe (MRS) media at 37°C for 48 h. The isolated strains were characterized with Gram staining, oxidase...
Effect of bacteriocins from lactic acid bacteria obtained from Zea mays-based “Ogi” on foodborne bacteria from contaminated cabbage
August 2021
The objective of this work was to evaluate the effect of bacteriocins from lactic acid bacteria (LAB) in Zea mays-based Ogi on some foodborne bacteria contaminating cabbage in Abakaliki, Nigeria. Ten (10) samples (5 samples of Z. mays-based Ogi and 5 samples of suspected contaminated cabbage heads) were aseptically collected and analyzed using standard microbiological methods. Five different Lactobacillus isolates (A,...
Phenotypic and genotypic characterization of antibiotic resistant gram negative bacteria isolated in Tabuk City, Saudi Arabia
August 2021
Antimicrobial surveillance and identifying the genetic basis of antimicrobial resistance provide important information to optimize patient care. The present study is an analytical cross sectional study aimed to determine the prevalence of multidrug resistant (MDR), extensively drug resistant (XDR), pan drug resistant (PDR) and extended-spectrum ?-lactamases genes among Gram-negative bacteria isolated in Saudi...
Heavy metals and microbial contamination of palm oil produced and sold at some markets in Kogi East Area, Kogi State, Nigeria
August 2021
The physicochemical, microbial and heavy metal contamination of palm oil samples randomly selected from three different markets (Ankpa, Anyigba and Idah Markets) in Kogi State, Nigeria, was investigated. The results revealed the presence of moisture (0.5, 0.4, 0.4%); impurity (0.4, 0.4, 0.3%) and free fatty acid (13, 12.8, 12.3%) for Ankpa, Anyigba and Idah Markets respectively. The microbial contaminants isolated were...
Prevalence and characterization of extended-spectrum ?-lactamase-producing Escherichia coli and Klebsiella pneumoniae isolated from poultry in Ouagadougou, Burkina Faso
August 2021
This study aimed to determine the prevalence of extended spectrum beta-lactamase producing (ESBL) and multidrug resistant Escherichia coli and Klebsiella pneumoniae. A cross-sectional study was conducted in Ouagadougou in two poultry farms and two slaughterhouses. 375 cloacal swabs and 46 environment samples were collected and members of Enterobacteriaceae were isolated on EMB agar containing 4 µg/L of cefotaxime....
Long-term effectiveness of commonly used hospital disinfectants against Pseudomonas aeruginosa and Staphylococcus aureus
July 2021
Nosocomial infections are enhanced due to a flop in the infection control processes such as disinfection. The aim of this study was to assess long term effectiveness and the stability of disinfectants currently used within healthcare settings in Accra, Ghana against two indicator pathogens. Two locally produced and two imported disinfectants usually used in health care settings were obtained. The efficacy and long-term...
Assessment of the physicochemical qualities and microbiological profile of Idah River, Kogi State, Nigeria
July 2021
Analysis of water bodies, such as rivers and lakes can provide an insight into their intrinsic composition and potential impact on the environment. Water samples collected from four designated sites in Idah River, were assessed for their physicochemical parameters and microbial diversity using standard procedures. The temperature from all sites was 26.00°C and the pH ranged from 6.93 to 7.08. Turbidity values ranged...
Isolation, identification and antibiogram of verotoxin producing Escherichia coli from raw salad vegetables at Jashore, Bangladesh
July 2021
Verotoxin-producing E. coli are getting an emergence for human health and day by day, it is also becoming more alarming in the fact that, they are acquiring multidrug-resistant profiles. This study aimed at isolation and identification of verotoxigenic E. coli followed by the antibiogram of the species from salad vegetables at Jashore district in Bangladesh. A total of 119 raw vegetable salad samples were collected from...
Molecular diagnosis of multidrug-resistant tuberculosis from culture-positive isolates using line probe assay in North Karnataka
July 2021
The control of tuberculosis (TB) has become a global health challenge due to the emergence of multidrug-resistant tuberculosis (MDR-TB) in Mycobacterium tuberculosis (MTB). This highlights the need for faster and more accurate detection of tuberculosis cases. The study aims to detect MDR-TB strains of pulmonary tuberculosis using resistance ratio method and to compare the diagnostic value of drug susceptibility...
Antibiotic susceptibility and production of extended-spectrum beta-lactamase (ESBL) of E. coli strains isolated from meat
July 2021
The zoonotic potential of Escherichia coli from chicken and beef food products is well documented. The uses of antibiotics on agriculture encourage the development of resistance bacteria capable of causing human disease and passing resistance to human pathogens. This study aimed to detect the antibiotic susceptibility and production of extended-spectrum beta-lactamase (ESBL) of E. coli strains isolated from meat. E....
Partial chemical characterization of the yeast extracts Lachancea thermotolerans CCMA 0763
July 2021
The fungi are living organisms of great importance around agriculture, food industry, and the pharmaceutical industry. The usage of these microorganisms in agriculture is for the biocontrol of diseases in plants of commercial interest. Some yeasts can fight phytopathogens through secondary metabolites produced, inhibiting their development. Some species show positive results in the control of pathologies in...
Antifungal and antibacterial activity of the different parts of mature Benincasa hispida against various microbial infectious agents
July 2021
In this study we assayed on antimicrobial activity of peel, pulp, waxy coating of Benincasa hispida. Various parts of the plant are reported as antibacterial agents worldwide. Leaves, flower, root, seed are the most studied elements as antibacterial agent. Some fruits are also proved as antibacterial agent and used as herbal medicine as well as nutritional supplements during disease. Different parts of mature and...
Distribution patterns of Vibrionaceae abundance on the landing stages in coastal area: Understanding the influence of physicochemical variables by using multiple linear regression models and corrgram for matrix correlation
June 2021
The present work used multiple linear regression (MLR) models and corrgram to assess the importance of environmental parameters on diversity and abundance dynamics of Vibrio sp. in waters of few landing stages in the city of Douala (Cameroon). It was recorded in all the five selected stations, the presence of four species of Vibrio namely, Vibrio parahaemolyticus, Vibrio cholerae, Vibrio fluvialis and Vibrio...
Shelf stability of processed cocoyam flour during storage at room temperature (28.0 ± 2°C) for a period of four months
June 2021
The shelf life study of dry milled cocoyam flour packaged in low density polyethylene was carried out for a period of 4-month. Microbiological, nutritional, physicochemical quality characteristics and aflatoxin content were evaluated. The total viable bacterial counts ranged from 1.6×103 - 4.8×105 cfu/g while the total viable fungal count increased from 5.0 × 101 - 3.8 × 105 sfu/g. The...
Substitution of non-biodegradable plastic food packagings by ecological food packagings at Abomey-Calavi University (Benin): State of place and microbiological quality of packagings
June 2021
Challenging with environmental problems and health due to non-biodegradable plastic wastes, Abomey-Calavi university heads have banned the use of any Non-biodegradable Plastic Food Packagings (NPFP) on all campuses. This study aimed to verify the effective implementation of this decision and assess the microbiological quality of ecological primary food packagings used in Abomey-Calavi campus. To achieve the...
Monitoring of physicochemical and microbiological parameters during nonnonkoumou (artisanal curdled milk) production in Daloa, Côte d’Ivoire
June 2021
This study aimed to study physicochemical and microbiological parameters during nonnonkoumou production. A total of 15 samples were analyzed during this study at a rate of 3 samples per fermentation time (T0 h, T6 h, T12 h, T18 h and T24 h). The physicochemical analyses showed a drop in pH (from 7.03 ± 0.028 to 4.59 ± 0.021) during the 24 h of fermentation. The titratable acidity increased from 0.30...
Prevalence and factors associated with Staphylococcus aureus nasal colonisation in orthopaedic patients at a tertiary care hospital in Kenya
June 2021
Staphylococcus aureus is an important organism in orthopaedic practice as it is the most common cause of orthopaedic infections including surgical site infections (SSIs), osteomyelitis and septic arthritis. Carriers of S. aureus are predisposed to developing invasive staphylococcal infections. Knowledge of a patient’s carrier status before surgery together with interventions to eliminate the carrier state have...
Probiotic properties of Lactobacilli isolated from human milk and their inhibitory effect on gastrointestinal pathogenic bacteria
June 2021
Breast feeding has critical effects on the newborns and either mother’s health. Some of such health-improving effects of the mother’s milk is associated with the beneficial microbes, lactic acid bacteria (LAB), which are normally present in the mother’s milk. Thus, human milk-associated lactobacilli were isolated in this study and some of their probiotic properties was investigated. Afterwards,...
Resistance profile of Salmonella isolated from food sold in the streets of N'Djamena, Chad
June 2021
The preparation and sale of street foods is booming in African cities in general and especially in N’Djamena. However, the hygienic failures observed during this activity constitute a source of contamination of these foods. The objective of this work is to determine the different serotypes of Salmonella isolated from food sold in the streets of N’Djamena in Chad and to test their sensitivity...
Screening for the prevalence of Helicobacter pylori infection among dyspeptic patients using simple fecal antigen and serum antibody diagnostic methods at Mukalla city Hospitals, Hadhramout, Yemen
June 2021
Helicobacter pylori is the most common infection in the world, and the most main causes of dyspepsia are related to H. pylori infection. In that respect, several non-invasive methods for the diagnosis of H. pylori infection were utilized. The current study aimed to determine the prevalence of H. pylori infection and its associated variables among dyspeptic patients in Mukalla city, Hadhramout-Yemen during a period from...
Molecular characterization of carbapenem resistant Gram-negative rods in Neonatal Intensive Care Unit of Mansoura University Children's Hospital
June 2021
Carbapenems are group of extended-spectrum β-lactam antimicrobials frequently used for treating multidrug-resistant Gram-negative bacilli (GNB) infections. This study aimed at detecting and characterizing carbapenem resistance (CR) genes among GNB isolated from patients treated in neonatal intensive care unit (NICU) of Mansoura University Children's Hospital (MUCH), Egypt. It is a prospective study conducted...
Characterization of diversity and pathogenecity of Pyricularia grisea affecting finger millet in Kenya
May 2021
Pyricularia grisea characterization is a prerequisite for species differentiation and understanding of the pathosystem, evolution and diversity of species. The aim of this study is to determine the morphological variation, pathogen virulence and molecular diversity of P. grisea isolates. Five isolates from infected heads of finger millet were collected from Bomet, Nakuru, Baringo, Busia and Machakos counties in 2019....
Evaluation of the efficacy of Trichoderma and Pseudomonas species against bacterial wilt Ralstonia isolates of tomato (Lycopersicum species)
May 2021
Ralstonia solanacearum causes bacterial wilt of tomato and limits the crop production, and antagonistic microorganisms use to suppress the disease, of which Trichoderma and Pseudomonas species are the most effective agents to control bacterial wilt. In the present study, attempt was made to isolate these two microorganisms to evaluate their effectiveness to control R. solanacearum, the causal agent of bacterial wilt...
Phenotypic and molecular characterization of multiple-resistant gram-negative bacteria in urine of pregnant women attending antenatal clinic of Mother and Child hospital, Ondo, Nigeria
May 2021
Phenotypic and molecular characterization of multiple antibiotic resistant Gram-negative bacteria in urine samples of pregnant women in Mother and Child Hospital, Nigeria was reported. In the study, 407 apparently healthy pregnant women were recruited. Structured questionnaire was administered to the patients to obtain their socio-demographic information and the medical history. Urine samples were collected, processed...
Isolation and in vitro screening of plant growth-promoting rhizobacteria from Solanum lycocarpum St. Hil., an endemic plant of the Brazilian tropical savannah
May 2021
Solanum lycocarpum St. Hil. is an endemic plant of the Brazilian tropical savannah (cerrado) that is capable of growing on acidic and nutrient-poor land, an ability which attracts attention to its rhizospheric microbiota, including plant growth-promoting rhizobacteria (PGPR). In this work, 131 bacterial strains were isolated from rhizosphere samples of S. lycocarpum and were tested in vitro for direct mechanisms of...
Role of heavy metals on antibiotic resistance properties in lactose fermenting isolates collected from textile industry effluents
May 2021
It is crucial to monitor the microbial ambience in heavy metal enriched industrial effluents that generally discharge into the environment. Therefore, the present study aimed to understand the load of heavy metals in multidrug-resistant lactose fermenting microorganisms isolating from textile industry effluents. Samples were collected from five different textile industrial canals. In most of the cases, the colony...
Role of mass gatherings in transmission of respiratory tract infections caused by Haemophilus influenzae
May 2021
Respiratory infection is one of the infections that can be transmitted in closed places due to the ease of transmission of pathogens from infected people to healthy people through droplets from coughing and sneezing, among these bacterial causes, Haemophilus influenza. Therefore, this study aimed to evaluate the prevalence of this type of bacteria among pilgrims coming from different geographical locations in the world...
Antibacterial activity of sweet orange (Citrus sinensis) juice extract on selected bacteria
April 2021
Plants have potentials to be developed into many new drugs yet to be discovered because of the countless chemical compositions in them. The investigation is targeted at the antibacterial activity of sweet orange juice extract on some bacteria using ethanol and ethyl ethanoate solvent to extract juice. Ditch method was used for the sensitivity testing against Escherichia coli, Staphylococcus aureus, Klebsiella pneumoniae...
Multidimensional aspects of surgical site infections due to non-tuberculous mycobacteria
April 2021
We read the recent publication on “Surgical site infections” (SSI) by Braga et al. (2021) with great interest. Moreover this paper has sensitized the surgeons, practitioners, microbiologists and quality managers on the need to look for non-tuberculous mycobacteria (NTM) as a cause for SSIs. Though NTM is one another cause for SSIs, and scientific articles and case reports including the present...
A prospective study of bacterial isolates profile in infected open fractures
April 2021
The most common complication in open fractures is infection, which often escalates to sepsis, osteomyelitis, and amputations. The use of prophylactic antibiotics is one of the most effective strategies to prevent infection. The prevailing bacterial isolate patterns must guide the choice of antibiotics for both prophylactic and empiric therapy. This study aims to describe the bacterial isolate profiles in infected open...
Molecular characterization of water-borne multi-drug resistant Escherichia coli
April 2021
Antibiotics are very important in the fight against infectious disease caused by bacteria and other microbes for decades. Today microbes have developed ways to resist antimicrobial agents targeted at them. We sought to characterize and simultaneously detect virulence genes associated water-borne antibiotic resistant Escherichia coli, obtained from water sampled from ground and surface water sources. The Analytical...
Virulence and multi-resistance of gram-negative bacilli strains isolated from some artisanal fermented dairy products sold in secondary schools in Benin
April 2021
This work aims at assessing toxin production capability and antibiotic resistance profiles of thermo-tolerant Gram-negative bacilli strains isolated from three types of fermented dairy products (yoghurt, dèguè millet and dèguè couscous). Samples collected in Abomey-Calavi and Cotonou were analyzed for microbial, biochemical and molecular parameters. Samples were contaminated with...
Bacteriological characteristics and resistance profiles of Vibrio cholerae O1 strains isolated in the Republic of Benin in 2020
April 2021
This study aims to carry out a bacteriological characterization and determine the resistance profile of Vibrio cholerae O1 strains isolated during the epidemiological season of 2020 in Benin. To achieve this goal, 43 diarrheal stool samples were analyzed. The samples were taken during the epidemic period of 2020. Bacteriological analyses consisted of enrichment of the samples in buffered peptone water followed by...
Evaluation of factors fuelling global antimicrobial resistance and its economic and clinical burden
March 2021
The aim of the research is to assess the factors fuelling Antimicrobial Resistance (AMR) and its economic and clinical burden. For this purpose, a systematic review was conducted, which included an analysis of factors and economic and clinical burden of AMR. It was found that poorly treated sewage; discharges from antibiotic manufacturing plants, empirical prescribing, lack of antimicrobial stewardship, poor AMR...
Antimicrobial activities of microbially-synthesized silver nanoparticles against selected clinical pathogens in Akure, Nigeria
March 2021
Silver nanoparticles (AgNPs) have stimulated interest of scientists due to their wide range of applications, including their potential antimicrobial activity. This study investigated the antimicrobial activities of AgNPs synthesized by rhizospheric soil and fish pond sediment microorganisms against selected clinical pathogens. The samples were cultured and organisms identified in accordance with standard procedures. The...
Anti-quorum sensing and anti-biofilm activities of Securidaca longepedunculata Fresen, an endangered species from Burkina Faso
March 2021
The emergence of bacterial resistance to antibiotics is a serious challenge to the global health system. The QS inhibition is one of the approaches to fight against antibiotic resistance in bacteria. Securidaca longepedunculata is a medicinal plant that roots are the only organ used against microbial diseases. This species is threatened with extinction due to the massive use of its roots in phytotherapy. In this study,...
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